BONE-MARROW OSTEOGENIC STEM-CELLS - INVITRO CULTIVATION AND TRANSPLANTATION IN DIFFUSION-CHAMBERS
BONE-MARROW OSTEOGENIC STEM-CELLS - INVITRO CULTIVATION AND TRANSPLANTATION IN DIFFUSION-CHAMBERS
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DOI：
10.1111/j.1365-2184.1987.tb01309.x
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发表时间：
1987-05-01
期刊：
影响因子：
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通讯作者：
GERASIMOV, UV
中科院分区：
文献类型：
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作者：
FRIEDENSTEIN, AJ;CHAILAKHYAN, RK;GERASIMOV, UV
通过解释骨髓单细胞悬液获得成纤维细胞集落（克隆），并通过合并集落或单个集落的连续传代，用于建立多集落和单集落衍生的成纤维细胞培养物。在体外培养20-30个细胞后，将成纤维细胞集落形成细胞（FCFC）的后代移植到扩散室中，无论是从单个集落还是混合集落生长，都保留了骨和软骨形成的能力。成骨前体细胞的含量在培养的后代显着超过初始FCFC。因此，骨髓FCFC的高增殖潜力和它们作为骨和软骨形成细胞的共同前体的能力使它们成为成骨干细胞作用的可能候选者。
Fibroblast colonies (clones) were obtained by explanation of bone marrow single-cell suspensions and were used to establish multicolony and single-colony derived fibroblast cultures by successive passaging of either pooled or individual colonies. When transplanted in diffusion chambers after 20-30 cell doublings in vitro, the descendants of fibroblast colony-forming cells (FCFC), whether grown from single or pooled colonies, retained the ability for bone and cartilage formation. The content of osteogenic precursors in the cultured progeny significantly outnumbered the initiating FCFC. Thus the high proliferative potential of bone marrow FCFC and their ability to serve as common precursors of bone and cartilage-forming cells makes them probable candidates for the role of osteogenic stem cells.