Macro-/nanoporous silicon as a support for high-performance protein microarrays
Macro-/nanoporous silicon as a support for high-performance protein microarrays
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DOI：
10.1021/ac034425q
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发表时间：
2003-12-15
影响因子：
7.4
通讯作者：
Laurell, T
中科院分区：
文献类型：
--
作者：
Ressine, A;Ekström, S;Laurell, T
目前的工作证明了使用大孔硅作为高敏感蛋白质芯片应用的衬底的可能性。通过电化学溶解单晶硅实现了三维多孔硅结构的形成。所制备的大孔硅网络具有刚性的海绵状结构，具有很高的均匀性和机械稳定性。底物的微流体特性被发现是良好的生物测定性能所必需的。斑点面积小，斑点重复性好，斑点轮廓均匀。测量了多孔表面的水接触角，并与平面硅、硅烷化玻璃和普通显微镜玻片的接触角进行了比较。介绍了多孔表面对免疫球蛋白结合免疫分析模型性能的影响。将aRIg G微分散到芯片表面，形成与目标分析物具有高亲和力的斑点微阵列。分配是使用内部开发的压电式流通式分配器进行的。每个斑点都是由每个位置的一个液滴(100Pl)形成的。大孔硅允许在人体血浆样品中进行高密度微阵列，点密度高达4400点/厘米(2)，没有串扰，仅消耗0.6pmoL抗体/1-cm(2)阵列。检测到低至70 pm的抗原水平。
The present work demonstrates the possibilities of using macroporous silicon as a substrate for highly sensitive protein chip applications. The formation of 3D porous silicon structures was performed by electrochemical dissolution of monocrystalline silicon. The fabricated macroporous silicon network has a rigid spongelike structure showing high uniformity and mechanical stability. The microfluidic properties of the substrates were found to be essential for a good bioassay performance. Small spot area, good spot reproducibility, and homogeneous spot profiles were demonstrated on the substrates for immobilized aRIgG. Water contact angles were measured on the porous surface and compared to that of planar silicon, silanized glass, and ordinary microscope glass slides. The effect of the porous surface on the performance of a model IgG-binding immunoassay is presented. aRIgG was microdispensed onto the chip surface forming a microarray of spots with high affinity for the target analyte. The dispensing was performed using an in-house-developed piezoelectric flow-through dispenser. Each spot was formed by a single droplet (100 pL) at each position. The macroporous silicon allowed a high-density microarraying with spot densities up to 4400 spots/cm(2) in human plasma samples without cross-talk and consumption of only 0.6 pmol of antibodies/1-cm(2) array. Antigen levels down to 70 pM were detected.