Pyocyanin Restricts Social Cheating in Pseudomonas aeruginosa.
Pyocyanin Restricts Social Cheating in Pseudomonas aeruginosa.
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DOI：
10.3389/fmicb.2018.01348
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发表时间：
2018
影响因子：
5.2
通讯作者：
García-Contreras R
中科院分区：
文献类型：
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作者：
Castañeda-Tamez P;Ramírez-Peris J;Pérez-Velázquez J;Kuttler C;Jalalimanesh A;Saucedo-Mora MÁ;Jiménez-Cortés JG;Maeda T;González Y;Tomás M;Wood TK;García-Contreras R
铜绿假单胞菌的群体感应(QS)协调毒力因子的表达，如外源蛋白水解酶和铁载体，这些是所有细菌种群利用的公共产品，无论他们是否投资于自己的生产。这些公共产品可以被QS缺陷突变体用来生长，由于这些突变体不会对公共产品生产做出贡献，他们被认为是社会骗子。绿青素是一种吩嗪，是铜绿假单胞菌产生的一种受QS控制的有毒代谢物。它是一种氧化还原活性化合物，能促进活性氧物种的生成；它还具有抗菌性能，并能提高与其他细菌物种竞争的适合性。由于QS缺陷个体对氧化应激的耐受性较差，我们假设野生型种群产生的绿青素可以促进该细菌中功能QS系统的选择。在这里，我们使用竞争实验和数学模型证明，实际上，绿青素增加了精通QS的合作个体的适合度，并限制了社会作弊者的出现。此外，我们还发现绿青素能够在鲍曼不动杆菌等其他细菌中选择QS。
Quorum sensing (QS) in Pseudomonas aeruginosa coordinates the expression of virulence factors, such as exoproteases and siderophores, that are public goods utilized by the whole population of bacteria, regardless of whether they invested or not in their production. These public goods can be used by QS defective mutants for growth, and since these mutants do not contribute to public goods production, they are considered social cheaters. Pyocyanin is a phenazine that is a toxic, QS-controlled metabolite produced by P. aeruginosa. It is a redox-active compound and promotes the generation of reactive oxygen species; it also possesses antibacterial properties and increases fitness in competition with other bacterial species. Since QS-deficient individuals are less able to tolerate oxidative stress, we hypothesized that the pyocyanin produced by the wild-type population could promote selection of functional QS systems in this bacterium. Here, we demonstrate, using competition experiments and mathematical models, that, indeed, pyocyanin increases the fitness of the cooperative QS-proficient individuals and restricts the appearance of social cheaters. In addition, we also show that pyocyanin is able to select QS in other bacteria such as Acinetobacter baumannii.