Culture, isolation, and labeling of Anaplasma phagocytophilum for subsequent infection of human neutrophils.
Culture, isolation, and labeling of Anaplasma phagocytophilum for subsequent infection of human neutrophils.
复制标题
培养、分离和标记嗜吞噬细胞无形体，用于随后感染人中性粒细胞。
DOI：
10.1007/978-1-60327-032-8_13
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
Borjesson,DoriL
中科院分区：
文献类型：
--
作者：
Borjesson,DoriL
Anaplasma phagocytophilumis the etiologic agent of granulocytic anaplasmosis, a tick-borne, zoonotic, emerging infectious disease.A. phagocytophilumis an obligate intracellular pathogen that primarily resides within membrane-bound, cytoplasmic vacuoles of host neutrophils. Closely related toEhrlichialandRickettsialorganisms,A. phagocytophilumis a small, fragile, Gram-negative bacterium that presents unique challenges for culture, isolation, enumeration, and labeling. This chapter delineates pathogen-specific considerations for culture and labeling of this organism for subsequent use in assays to examine mechanisms of host cell–pathogen interactions.
