New ways of imaging uptake and intracellular fate of liposomal drug carrier systems inside individual cells, based on Raman microscopy
New ways of imaging uptake and intracellular fate of liposomal drug carrier systems inside individual cells, based on Raman microscopy
复制标题
DOI：
10.1021/mp7001158
复制
发表时间：
2008-03-01
影响因子：
4.9
通讯作者：
Diem, Max
中科院分区：
文献类型：
--
作者：
Matthaeus, Christian;Kale, Amit;Diem, Max
最近的发展，结合拉曼光谱与光学显微镜，提供了一种新的非侵入性技术，以评估和图像的细胞过程。特别感兴趣的是各种细胞学活性化合物的摄取机制。为了在光谱学上区分感兴趣的物种与它们的细胞环境，化合物可以用氘标记。在这里，我们应用拉曼显微光谱学来跟踪脂质体药物载体系统的摄取，该系统已被引入以将生物活性化合物递送至其在人乳腺癌MCF-7细胞内的作用部位。脂质体和脂质体表面修饰的细胞穿透肽（TAT-肽，TATp）的分布模式已随时间成像。获得的光谱信息提供了一个明确的证据，可变的速率，以及不同的效率的脂质体摄取取决于它们的表面性质。根据实验设置，该技术可应用于固定或活细胞生物体。
Recent developments, combining Raman spectroscopy with optical microscopy, provide a new noninvasive technique to assess and image cellular processes. Of particular interest are the uptake mechanisms of various cytologically active compounds. In order to distinguish the species of interest from their cellular environment spectroscopically, compounds may be labeled With deuterium. Here, we apply Raman microspectroscopy to follow the uptake of liposomal drug carrier systems that have been introduced to deliver biologically active compounds to their site of action within human breast adenocarcinoma MCF-7 cells. The distribution patterns of liposomes and liposomes surface-modified with a cell-penetrating peptide (TAT-peptide, TATp) have been imaged over time. The spectroscopic information obtained provides a clear evidence for variable rates, as well as different efficiencies of liposome uptake depending on their surface properties. Depending on the experimental setup, the technique may be applied to fixed or living cell organisms.