Distribution of morphinan and benzo[c]phenanthridine alkaloid gene transcript accumulation in Papaver somniferum
Distribution of morphinan and benzo[c]phenanthridine alkaloid gene transcript accumulation in Papaver somniferum
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DOI：
10.1016/s0031-9422(99)00600-7
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发表时间：
2000-03-01
期刊：
影响因子：
3.8
通讯作者：
Kutchan, TM
中科院分区：
文献类型：
--
作者：
Huang, FC;Kutchan, TM
罂粟Papaver somniferum L.产生抗菌的苯并[c]菲啶生物碱血根碱和麻醉镇痛的吗啡烷生物碱吗啡。通过RNA凝胶印迹分析，研究了3个生物碱生物合成基因在罂粟幼苗、成熟植株和悬浮培养细胞中的转录水平，以及茉莉酸甲酯诱导的生物碱积累。它们编码吗啡和血根碱生物合成中共有的（S）-N-甲基乌药碱3 '-羟化酶（CYP 80 B1）、位于血根碱合成途径上的小檗碱桥酶（BBE）和吗啡生物合成的倒数第二个酶可待因酮还原酶（COR）。在发育中的罂粟幼苗中，吗啡前体蒂巴因在整个萌发的前20天都存在。与此相反，血根碱仅在发芽后第五天才以可检测的量存在，并且至少持续增加到第二十天。cyp 80 b1、bbe 1和cor 1基因转录本的积累掩盖了这些差异。在成熟罂粟植株中，cyp 80 b1、bbe 1和cor 1基因在根、茎、叶片和叶中脉中均有表达。而在花蕾和蒴果中只检测到cup 80 b1和cor 1。与血根碱积累，而不是吗啡积累，可以诱导罂粟细胞悬浮培养中加入茉莉酸甲酯的培养基，cyp 80 b1和bbe 1，但不是cor 1转录积累响应激发子处理的事实相一致。(C)2000爱思唯尔科技有限公司版权所有。
The opium poppy Papaver somniferum L. produces the antimicrobial benzo[c]phenanthridine alkaloid sanguinarine and the narcotic analgesic morphinan alkaloid morphine. Transcripts of three genes of alkaloid biosynthesis in P. somniferum in developing seedlings, mature plants and plant cell suspension culture were monitored for temporal/spatial or for methyl jasmonate-induced accumulation by RNA gel blot analysis. These gents encoded (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1) that is common to morphine and sanguinarine biosynthesis, the berberine bridge enzyme (BBE) that lies on the pathway to sanguinarine, and codeinone reductase (COR) the penultimate enzyme of morphine biosynthesis. In developing P. somniferum seedlings, the morphine precursor thebaine was present throughout the first twenty days of germination. In contrast, sanguinarine was present in detectable quantities only after day five after germination and continued to increase at least until day twenty. Accumulation of cyp80b1 bbe1 and cor1 gene transcripts paralleled these differences. In the mature poppy plant, cyp80b1, bbe1 and cor1 gene transcripts were detected in the root, the stem, the leaf lamina and the leaf mid rib. Only cup80b1 and cor1, however, were found in the flower bud and the capsule. Consistent with the fact that sanguinarine accumulation, but not that of morphine, can be induced in opium poppy cell suspension culture by addition of methyl jasmonate to the culture medium, cyp80b1 and bbe1, but not cor1 transcript accumulated in response to elicitor treatment. (C) 2000 Elsevier Science Ltd. All rights reserved.