Postnatal development of nicotinamide adenine dinucleotide phosphate‐diaphorase‐positive neurons in the retina of the golden hamster
Postnatal development of nicotinamide adenine dinucleotide phosphate‐diaphorase‐positive neurons in the retina of the golden hamster
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DOI：
10.1002/cne.10201
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发表时间：
2002-05
影响因子：
2.5
通讯作者：
D. Tay;Y. Diao;Yue-mei Xiao;K. So
中科院分区：
文献类型：
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作者：
D. Tay;Y. Diao;Yue-mei Xiao;K. So
采用组织化学方法研究金黄地鼠视网膜内烟酰胺腺嘌呤二核苷酸磷酸黄递酶（NADPH-d）阳性神经元的生后发育。在出生后第1天（P），在视网膜中可辨别出NADPH-d阳性神经元。从P4开始到成年，当视网膜获得其层状特征时，在内核层（INL）和神经节细胞层（GCL）中观察到NADPH-d阳性神经元。结果表明，INL和GCL中的NADPH-d-阳性神经元在其发育过程中遵循不同的时间过程和模式。INL中的NADPH-d阳性神经元从P4到P8急剧增加（3.6倍），随后在P12时降至最大值的46%。这一数值相对于成人水平保持不变。INL中NADPH-d阳性神经元的平均直径在所有年龄段均小于GCL中的平均直径，从P8到P12和从P20到成年期增加。至于GCL中的神经元，在出生后的早期，直到P20，细胞数量的增加不是那么明显，此后，观察到一个明显的增加到成人水平。GCL中NADPH-d阳性细胞体的平均直径随年龄增加而增加，但P16-P20除外，在此期间有轻微且不显著的减少。INL和GCL的细胞密度变化趋势与总数变化趋势基本一致。在P12 ~ P20之间，NADPH-d-阳性神经元的密度分布图发生了显著变化：最高密度从出生早期的上中央视网膜转移到成年后的下中央视网膜。NADPH-d阳性神经元的两波增加与轴突伸长和突触发生以及获得视觉功能和经验的过程相一致。这表明这些NADPH-d-阳性神经元与这两个发育事件有关。J. Comp.神经元446：342-348，2002.© 2002 Wiley利斯公司
The histochemical method was used to investigate the postnatal development of nicotinamide adenine dinucleotide phosphate‐diaphorase (NADPH‐d) ‐positive neurons in retinas of the golden hamster. NADPH‐d‐positive neurons were discernible in the retina at postnatal day (P)1. From P4 onward to adulthood, when the retina acquired its laminated characteristics, NADPH‐d‐ positive neurons were observed in the inner nuclear layer (INL) and the ganglion cell layer (GCL). Results showed that NADPH‐d‐positive neurons in INL and GCL followed different time courses and patterns in their development. NADPH‐d‐positive neurons in INL underwent a sharp increase from P4 to P8 (3.6‐fold), followed by a decrease to 46% of the maximum at P12. This value was maintained relatively constant to the adult level. The mean diameters of NADPH‐d‐positive neurons in INL, which were smaller than those in the GCL for all ages, increased from P8 to P12 and from P20 to adulthood. As for neurons in the GCL, the increase in cell number was not so apparent for the earlier postnatal days until P20; thereafter, an obvious increase to the adult level was observed. The mean diameters of the NADPH‐d‐positive cell bodies in the GCL increased with age, except for P16–P20, during which time there was a slight and insignificant decrease. The tendency of changes in cell density was basically similar to that of the total number for both the INL and the GCL. Between P12 and P20, the density distribution map of the NADPH‐d‐positive neurons underwent dramatic changes: The highest density shifted from the upper central retina at the earlier postnatal days to the lower central retina in the adult. The two waves of increase in NADPH‐d‐positive neurons coincide with the process of axonal elongation and synaptogenesis and the acquisition of visual function and experience. It is suggested that these NADPH‐d‐positive neurons are related to these two developmental events. J. Comp. Neurol. 446:342–348, 2002. © 2002 Wiley‐Liss, Inc.