Effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver.
Effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver.
复制标题
妊娠后期限制采食量对绵羊肝脏功能、抗氧化能力及急性期蛋白合成的影响
DOI：
10.5713/ajas.18.0331
复制
发表时间：
2019-03
期刊：
影响因子：
--
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Yang H;Wang Y;Ma C;Sun C;Liu Y;Wu K;Li M;Borjigin G;Gao F
本试验旨在研究妊娠后期限制采食量对绵羊肝脏功能、抗氧化能力和急性期蛋白合成的影响。十八只具有单胎胎儿的交配母羊被分配到三组：限制组 1（RG1，0.18 MJ ME/kg W0.75 d，n = 6），限制组 2（RG2，0.33 MJ ME/kg W0.75 d），n = 6）和对照组（CG，随意采食，0.67 MJ ME/kg W0.75 d，n = 6).禁食期为妊娠90天至140天。限制组母羊体重、肝脏重量、水分和肝脏蛋白质含量均较CG组降低（p<0.05），但RG1组肝脏脂肪含量高于CG组（p<0.05）。随着能量摄入的减少，限制组中观察到肝胶原纤维和网状纤维增加。随着能量摄入的减少，RG1和RG2组的非酯化游离脂肪酸浓度高于CG组（p<0.05），但与CG组相比，两个限制组的脂蛋白脂肪酶和肝脂肪酶浓度均降低（p<0.05）。此外，与CG组相比，RG1组的β-羟基丁酸、甘油三酯、丙二醛、总抗氧化能力、超氧化物歧化酶活性和过氧化氢酶活性均升高，胆碱酯酶浓度降低（p<0.05）。急性时相蛋白浓度方面，RG1组C反应蛋白（CRP）较CG组降低，但触珠蛋白与对照组相比无差异（p>0.05）。妊娠后期母体营养不良导致母羊肝脏脂肪堆积、肝纤维化加剧、抗氧化失衡和急性期蛋白合成改变，不利于肝功能适应妊娠。
An experiment was conducted to investigate the effects of feed intake restriction during late pregnancy on the function, anti-oxidation capability and acute phase protein synthesis of ovine liver. Eighteen time-mated ewes with singleton fetuses were allocated to three groups: restricted group 1 (RG1, 0.18 MJ ME/kg W0.75 d, n = 6), restricted group 2 (RG2, 0.33 MJ ME/kg W0.75 d), n = 6) and a control group (CG, ad libitum, 0.67 MJ ME/kg W0.75 d, n = 6). The feed restriction period was from 90 days to 140 days of pregnancy. The ewe’s body weight, liver weights, water, and protein content of liver in the restricted groups were reduced compared with the CG group (p<0.05), but the liver fat contents in the RG1 group were higher than those of the CG group (p<0.05). The increased hepatic collagen fibers and reticular fibers were observed in the restricted groups with the reduction of energy intake. The concentrations of nonesterified free fatty acids in the RG1 and RG2 groups were higher than those of the CG group with the reduction of energy intake (p<0.05), but there were decreased concentrations of lipoprotein lipase and hepatic lipase in both restricted groups compared with the CG group (p<0.05). In addition, the increased concentrations of β-hydroxybutyric acid, triglycerides, malondialdehyde, total antioxidant capacity and activities of superoxide dismutase activity and catalase were found in the RG1 group, and the concentrations of cholinesterase in the RG1 group were reduced compared with the CG group (p<0.05). For the concentrations of acute phase proteins, the C-reactive protein (CRP) in the RG1 group were reduced compared with the CG group, but there were no differences in haptoglobin relative to the controls (p>0.05). The fat accumulation, increased hepatic fibrosis, antioxidant imbalance and modified synthesis of acute phase proteins were induced in ewe’s liver by maternal malnutrition during late pregnancy, which were detrimental for liver function to accommodate pregnancy.