Detection of fluid secretion of three‐dimensional reconstructed eccrine sweat glands by magnetic resonance imaging
Detection of fluid secretion of three‐dimensional reconstructed eccrine sweat glands by magnetic resonance imaging
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DOI：
10.1111/exd.13833
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发表时间：
2019-01
影响因子：
3.6
通讯作者：
Haihong Li;Liyun Chen;Mingjun Zhang;Sitian Xie;Cuiping Zhang
中科院分区：
文献类型：
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作者：
Haihong Li;Liyun Chen;Mingjun Zhang;Sitian Xie;Cuiping Zhang
我们之前展示了三维(3D)重建的汗腺与天然的汗腺具有相似的结构，但3D重建的汗腺是否适当地分泌液体仍是未知的。在本研究中，将Matrigel包埋的人汗腺细胞或Matrigel单独植入裸鼠的腹股沟皮下。植入后10周，用7.0T的脂肪抑制质子密度加权磁共振成像(MRI)序列采集注射匹罗卡品/生理盐水(NS)前后大鼠皮下形成的栓子和足垫的图像，并分析和绘制栓子和脚垫中的感兴趣区(ROI)。注射匹罗卡品后，3D重建汗腺和自然汗腺的ROI平均质子强度均显著增加。注射匹罗卡品前后Matrigel塞感兴趣区平均质子强度无明显变化，注射生理盐水前后大鼠足垫感兴趣区质子强度无明显变化。总之，3D重建的汗腺有液体分泌，这是通过脂肪抑制的质子密度加权MRI可以检测到的。
We previously showed three‐dimensional (3D) reconstructed eccrine sweat glands have similar structures as native eccrine sweat glands, but whether the 3D reconstructed sweat glands appropriately secrete fluid is still unknown. In this study, Matrigel‐embedded human eccrine sweat gland cells or Matrigel alone were implanted into the groin subcutis of the nude mice. Ten weeks post‐implantation, images of the subcutaneously formed plugs, as well as footpads of rats, pre‐ and post‐pilocarpine/normal saline (NS) injection were acquired using a fat‐suppressed proton density‐weighted magnetic resonance imaging (MRI) sequence at 7.0 T, and the regions of interest (ROIs) in plugs and rat footpads were analysed and graphed. A significant increase in the ROI mean proton intensity occurred in both 3D reconstructed and native eccrine sweat glands after pilocarpine injection. The mean proton intensity had no noticeable changes in ROIs of Matrigel plugs between pre‐ and post‐pilocarpine injection, and in ROIs of rat footpads between pre‐ and post‐NS injection. In conclusion, the 3D reconstructed sweat glands possess fluid secretion, which is detectable by fat‐suppressed proton density‐weighted MRI.