Application of the stoichiometric displacement model of retention to anion-exchange chromatography of nucleic acids.

Application of the stoichiometric displacement model of retention to anion-exchange chromatography of nucleic acids.
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保留的化学计量置换模型在核酸阴离子交换色谱中的应用。

DOI:
10.1016/0021-9673(86)80069-3
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发表时间:
1986
期刊:
Journal of chromatography
影响因子:
--
通讯作者:
Regnier,FE
Regnier,FE
中科院分区:
--
文献类型:
--
作者:
Drager,RR;Regnier,FE

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化学计量置换模型在阴离子交换色谱的应用中得到了改进。经修订的化学计量置换模型已被证明是有效的阴离子交换色谱相对于所有细节的模型测试。研究表明,使用置换剂活性并不是将化学计量置换模型有效应用于阴离子交换色谱的必要条件。虽然置换盐的阳离子可以影响阴离子交换色谱,但数据表明置换阴离子是最重要的。结果表明,具有三维结构的溶质Zn值与溶质电荷比小于1,化学计量置换模型可作为溶质三维结构的探针。
The stoichiometric displacement model has been refined in its application to anion-exchange chromatography. The revised stoichiometric displacement model has been shown to be valid for anion-exchange chromatography with respect to all particulars of the model tested. It has been shown that the use of displacing agent activity is not a necessary condition for valid application of the stoichiometric displacement model to anion-exchange chromatography. While the cation of the displacing salt can influence anion-exchange chromatography, the data indicate that the displacing anion is of primary importance. It has been shown that solutes with three-dimensional structure have Znvalue to solute charge ratios less than unity, and that the stoichiometric displacement model may be useful as a probe of solute three-dimensional structure.
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