Leucine pools in Escherichia coli biofilm discovered by Raman imaging
Leucine pools in Escherichia coli biofilm discovered by Raman imaging
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DOI：
10.1002/jrs.2952
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发表时间：
2011-11
影响因子：
2.5
通讯作者：
Hemanth Nag Noothalapati Venkata;N. Nomura;Shinsuke Shigeto
中科院分区：
文献类型：
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作者：
Hemanth Nag Noothalapati Venkata;N. Nomura;Shinsuke Shigeto
在被称为生物膜的结构化细菌群落中，各种生物分子在生物膜的形成中发挥着独特的信号和/或调节作用。在这里，我们报告了第一次通过拉曼成像在一个30小时前的大肠杆菌生物膜的微菌落内检测到高水平的氨基酸亮氨酸(亮氨酸池)。多频拉曼图像显示亮氨酸的定位表明，在大肠杆菌生物膜形成的早期阶段，亮氨酸积累，这可能是生理环境特异性代谢适应的结果。我们证明，我们的无标记拉曼成像方法提供了一个有用的平台，用于直接识别生物膜中产生的仍未知的天然产物，以及可视化原位生物膜组分的异质分布。版权所有©2011 John Wiley&Sons，Ltd.
In structured communities of bacteria known as biofilms, a variety of biomolecules have been shown to play a unique role as signals and/or regulators in biofilm formation. Here, we report that high levels of the amino acid leucine (leucine pool) were detected, for the first time, within microcolonies in a 30-h-old Escherichia coli biofilm by Raman imaging. Localization of leucine revealed by multifrequency Raman images indicates leucine accumulation during the early stage of the E. coli biofilm formation, which may have resulted from physiological environment-specific metabolic adaptation. We demonstrate that our label-free Raman imaging method provides a useful platform for directly identifying still unknown natural products produced in biofilms as well as for visualizing heterogeneous distributions of biofilm constituents in situ. Copyright © 2011 John Wiley & Sons, Ltd.