Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing
Identification of active aerobic methanotrophs in plateau wetlands using DNA stable isotope probing
复制标题
DNA稳定同位素探测鉴定高原湿地活性需氧甲烷氧化菌
DOI：
10.1093/femsle/fnw168
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发表时间：
2016-08-01
影响因子：
2.1
通讯作者：
Dumont, Marc G.
中科院分区：
文献类型：
--
作者：
Deng, Yongcui;Cui, Xiaoyong;Dumont, Marc G.
这些地点的甲烷氧化菌在减少甲烷排放中发挥作用，但对这些湿地中活性甲烷氧化菌的组成知之甚少。本文采用DNA稳定同位素探测技术，对高原三片莎草湿地中关键活性好氧甲烷氧化菌进行了鉴定。结果表明，红原和党雄两个泥炭地均有甲基藻属植物活跃。甲基杆菌只在党雄泥炭中有活性。红原泥炭的甲烷氧化速率最高，并且观察到甲烷营养型菌丝菌与甲烷营养型菌丝菌的碳交叉取食。由于孵育过程中甲烷氧化率较低，未对麻多湿地样品中甲烷氧化菌进行标记。结果表明，该地区湿地甲烷氧化菌的活性存在较大差异。
Sedge-dominated wetlands on the Qinghai-Tibetan Plateau are methane emission centers. Methanotrophs at these sites play a role in reducing methane emissions, but relatively little is known about the composition of active methanotrophs in these wetlands. Here, we used DNA stable isotope probing to identify the key active aerobic methanotrophs in three sedge-dominated wetlands on the plateau. We found that Methylocystis species were active in two peatlands, Hongyuan and Dangxiong. Methylobacter species were found to be active only in Dangxiong peat. Hongyuan peat had the highest methane oxidation rate, and cross-feeding of carbon from methanotrophs to methylotrophic Hyphomicrobium species was observed. Owing to a low methane oxidation rate during the incubation, the labeling of methanotrophs in Maduo wetland samples was not detected. Our results indicate that there are large differences in the activity of methanotrophs in the wetlands of this region.