NEW METHOD FOR DETECTION AND ENUMERATION OF MANGANESE OXIDIZING AND REDUCING MICROORGANISMS
NEW METHOD FOR DETECTION AND ENUMERATION OF MANGANESE OXIDIZING AND REDUCING MICROORGANISMS
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DOI：
10.1007/bf01611203
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发表时间：
1973-01-01
期刊：
影响因子：
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通讯作者：
ALTMANN, HJ
中科院分区：
文献类型：
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作者：
KRUMBEIN, WE;ALTMANN, HJ
1.本文研究了小檗林蓝-I（N，N ′-二甲氨基-p，p′-三苯基甲烷-o ″-磺酸）作为微生物培养基中锰氧化和锰还原的检测试剂。2.在微酸性溶液中的Berbelin蓝可以添加到培养基中，而不影响微生物的种群生长。通过出现深蓝色来检测Mn II溶液氧化为Mn III和IV。3.在长期培养实验中，最好在培养结束后再加入Berbelin blue-I溶液。4. Berbelin blue-I溶液与锰IV-VII在3.5和10之间的pH范围内反应，从而允许在期望的pH值下进行测试。5.微生物对锰VII或锰IV的还原可通过在培养期后加入柏贝林蓝-I溶液来检测。深蓝色介质中的白色晕圈表明Mn-IV化合物的还原优于在淡紫色或灰色介质中出现的白色斑点，如通过将氧化锰或高锰酸盐溶液掺入测试介质中所产生的。6.与Feigl's Benzidinium试剂相比，Berbelin-I溶液在研究船上的生态工作和定量工作更容易，危险性更小。7.在醋酸盐缓冲液中，测定锰和氧在618 nm处的显色光密度，可用于锰和氧的定量测定。
1. Berbelin blue-I (N.N.′-Dimethylamino-p, p′-triphenylmethane-o″-sulphonic acid) has been examined as a reagent for the detection of manganese oxidation and manganese reduction in microbial culture media. 2. Berbelin blue in slightly acid solution may be added to media without influencing population growth of micro-organisms. The oxidation of Mn II solutions to Mn III and IV is detected by occurrence of deep blue colours. 3. In case of long-term culture experiments, it is more advisable to add Berbelin blue-I solutions only after cultivation. 4. Berbelin blue-I solutions react with manganese IV–VII in pH ranges between 3.5 and 10, thus allowing tests at desired pH values. 5. Reduction of manganese VII or manganese IV by micro-organisms may be detected by addition of Berbelin blue-I solutions after cultivation periods. White halos within dark-blue coloured media indicate reduction of Mn-IV compounds better than occurrence of white spots in slightly violet or grey media, as produced by the incorporation of manganese oxides or permanganate solutions to test media. 6. Ecological work and quantitative work on board research vessels is much easier and less dangerous with Berbelin-I solutions than withFeigl's Benzidinium reagent. 7. Berbelin blue-I may be used for quantitative determination of manganese and oxygen when measuring optical density of the colour complex at 618 nm in wide concentration ranges and working in acetate buffer.