Phosphorylation of human erythrocyte band 3 by endogenous p72syk.
Phosphorylation of human erythrocyte band 3 by endogenous p72syk.
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DOI：
10.1016/s0021-9258(17)42204-6
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发表时间：
1994-01
期刊：
影响因子：
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通讯作者：
M. Harrison;C. Isaacson;Debra L. Burg;R. Geahlen;Philip S. Low
中科院分区：
文献类型：
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作者：
M. Harrison;C. Isaacson;Debra L. Burg;R. Geahlen;Philip S. Low
阴离子转运体，带3，是主要的酪氨酸激酶底物在红细胞膜和完整的人红细胞。利用各种蛋白酪氨酸激酶的抗体，我们发现p72syk和p56/53lyn存在于人类红细胞中，而p56lck、pp60src、p59fyn和p55blk不存在。用钒酸盐和过氧化氢的组合处理完整的红细胞显著增加了带3的酪氨酸磷酸化。在免疫复合物激酶试验中，该处理增加了p72syk的酪氨酸激酶活性，降低了p56/53lyn的活性。在免疫复合物中发现带3与p72syk相关，而与p56/53lyn无关。这些发现表明p72syk至少在一定程度上导致了人类红细胞中带3的酪氨酸磷酸化。
The anion transporter, band 3, is the major tyrosine kinase substrate in both red cell membranes and intact human erythrocytes. Using antibodies to various protein-tyrosine kinases, we found that p72syk and p56/53lyn are present in human red cells, while p56lck, pp60src, p59fyn, and p55blk are absent. Treatment of intact red cells with a combination of vanadate and hydrogen peroxide dramatically increased the tyrosine phosphorylation of band 3. This treatment increased the tyrosine kinase activity of p72syk and decreased the activity of p56/53lyn in immune complex kinase assays. Band 3 was found to be associated in immune complexes with p72syk but not with p56/53lyn. These findings suggest that p72syk is responsible, at least in part, for the tyrosine phosphorylation of band 3 in human erythrocytes.