The purification and the amino acid content of vasopressin preparations.
The purification and the amino acid content of vasopressin preparations.
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加压素制剂的纯化及氨基酸含量测定。
DOI：
10.1016/s0021-9258(18)50947-9
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发表时间：
1951
期刊：
影响因子：
--
通讯作者：
V. Vigneaud
中科院分区：
文献类型：
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作者：
R. Turner;John G. Pierce;V. Vigneaud
自从发现了脑下垂体后叶提取物的升压活性以来，许多实验室研究了从该腺体提取的提取物的分级。各种制剂的生理效力以及所采用的分级方法已经相当详细地进行了审查(24)。本文报道了一种纯化加压素的新方法，该方法得到了高效的制剂。并对这些制剂的氨基酸组成进行了研究。早期的研究表明，在高效的加压素制剂中存在某些氨基酸。1933年，据报道，一种制剂的效力为每毫克206个单位的升压活性。含有较多的酪氨酸和硫磺(5)。后来，Irving、Dyer和Du Vigneaud(6)使用电泳法纯化的材料，发现在初步纯化后，酪氨酸和胱氨酸的含量随着升压强度的增加而增加。在他们最有效的制剂中(每毫克200单位)，他们获得了9.9%(酪氨酸)和11.2%(胱氨酸)的值。Stehle和Frazer(7)描述了效力为每毫克200单位的制剂。它含有9.5%的酪氨酸、7.7%的胱氨酸和8.9%的精氨酸。他们报告说，他们的制剂中缺乏苯丙氨酸，谷氨酸、天冬氨酸和亮氨酸可能也缺乏。后来，Stehle和Trister(8)报告了他们的制剂中存在脯氨酸和异亮氨酸，而没有色氨酸、甘氨酸、组氨酸和羟脯氨酸。最近，波茨和加拉格尔(9)报道了一项准备工作-
Since the discovery (1) of the pressor activity of extracts of the posterior lobe of the pituitary gland, many laboratories have investigated the fractionation of the extracts obtained from this gland. The physiological potency of various preparations, as well as the methods of fractionation employed, have already been reviewed in considerable detail (24). In the present paper we report a new method of purification of vasopressin’which has yielded preparations of high potency. A study of the amino acid composition of these preparations is also presented. Earlier investigations have shown the presence of certain amino acids in vasopressin preparations of high potency. In 1933 it was reported that a preparation having a potency of 206 units of pressor activity per mg. contained a relatively large amount of tyrosine and of sulfur (5). Later Irving, Dyer, and du Vigneaud (6), using electrophoretically purified material, found that after preliminary purification the tyrosine and cystine content increased with the pressor potency. They obtained the values 9.9 per cent (tyrosine) and 11.2 per cent (cystine) in their most potent preparation (200 units per mg.). Stehle and Frazer (7) described preparations having a potency of 200 units per mg. which contained 9.5 per cent tyrosine, 7.7 per cent cystine, and 8.9 per cent arginine. They reported that phenylalanine was lacking in their preparations, and that glutamic acid, aspartic acid, and leucine were probably lacking. Later, Stehle and Trister (8) reported the presence of proline and isoleucine in their preparations and the absence of tryptophan, glycine, histidine, and hydroxyproline. More recently Potts and Gallagher (9) reported a prep-