Muscarinic receptor-mediated increase in zeta-PKC expression in SK-N-SH human neuroblastoma cells.
Muscarinic receptor-mediated increase in zeta-PKC expression in SK-N-SH human neuroblastoma cells.
复制标题
SK-N-SH 人神经母细胞瘤细胞中毒蕈碱受体介导的 zeta-PKC 表达增加。
DOI：
10.1007/bf00966807
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发表时间：
1994
影响因子：
4.4
通讯作者：
Dyer,KD
中科院分区：
文献类型：
--
作者：
Baumgold,J;Dyer,KD
Anti-peptide antibodies specific for each protein kinase C (PKC) isozyme were used to screen SKN-SH human neuroblastoma cells. These cells were found to express only α- and ζ-PKC. Stimulation of these cells with phorbol esters caused α-but not ζ-PKC to translocate from cytosolic to membrane fractions. Stimulation of these cells with carbachol, which releases inositol trisphosphate and diacylglycerol, caused a transient translocation of α-PKC but not of ζ-PKC. Carbachol did, however, cause a gradual increase in immunoreactive ζ-PKC which reached maximal values 10–20 min after stimulation. These results implicate ζ-PKC in a receptor-mediated signalling event.
