Interventional Optical Molecular Imaging Guidance during Percutaneous Biopsy
Interventional Optical Molecular Imaging Guidance during Percutaneous Biopsy
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DOI：
10.1148/radiol.14131880
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发表时间：
2014-06-01
期刊：
影响因子：
19.7
通讯作者：
Mahmood, Umar
中科院分区：
文献类型：
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作者：
Sheth, Rahul A.;Heidari, Pedram;Mahmood, Umar
目的：研究吲哚青绿(ICG)作为肝脏内恶性病变的分子信标，并评价所开发的手持成像系统在小鼠模型中以高目标背景比测量ICG荧光的能力。材料和方法：所有动物实验均经机构动物护理委员会批准。手持式光学分子成像装置用于穿透标准经皮活检试剂盒的引导针。建立了一个体外体模系统来定量ICG在肝实质中的组织衰减特性。随后在裸鼠体内建立肝内结直肠癌转移瘤模型，于移植后3周(n=6)行ICG荧光成像及肝组织学分析。于注射后3、6、24小时用手持成像设备对15例肝内结直肠转移瘤患者进行荧光成像。计算每个时间点的靶-背景比。随后，使用标准的临床18号活检针对肝内结直肠转移瘤进行核心活检。结果：ICG在所有时间点都能准确定位局灶性病变。同样，手持成像系统检测到的肿瘤内的荧光比正常肝脏内的荧光更强(24小时的平均目标背景比+/-标准差为3.9+/-0.2)。使用标准活检针对肿瘤和正常邻近肝脏进行核心活检，在肿瘤-肝脏界面显示出清晰的荧光强度边缘。结论：在小鼠肝内结直肠癌转移模型中，定制的分子成像设备与ICG相结合，可以很容易地区分正常和恶性组织。(C)RSNA，2014年
Purpose: To investigate indocyanine green (ICG) as a molecular beacon for malignant lesions within the liver and evaluate the ability of a developed handheld imaging system to allow measurement of ICG fluorescence within focal hepatic lesions with high target-to-background ratios in a mouse model.Materials and Methods: All animal experiments were approved by the institutional animal care committee. A handheld optical molecular imaging device was constructed to pass through the introducer needle of a standard percutaneous biopsy kit. An ex vivo phantom system was constructed to quantify tissue attenuation properties of ICG in liver parenchyma. Subsequently, intrahepatic colorectal cancer metastases were generated in nude mice, and epifluorescence imaging of ICG, as well as histologic analysis of the explanted livers, was performed at 3 weeks after implantation (n = 6). Epifluorescence imaging with the handheld imaging device was then performed on intrahepatic colorectal metastases after the administration of ICG (n = 15) at 3, 6, and 24 hours after injection. Target-to-background ratios were calculated for each time point. Subsequently, a core biopsy of intrahepatic colorectal metastases was performed by using a standard clinical 18-gauge biopsy needle.Results: There was avid localization of ICG to the focal lesions at all time points. Similarly, fluorescence within the tumors was greater than that within normal liver, as detected with the handheld imaging system (mean target-to-background ratio +/- standard deviation, 3.9 +/- 0.2 at 24 hours). A core biopsy of tumor and normal adjacent liver by using a standard biopsy needle demonstrated a sharp margin of fluorescence intensity at the tumor-liver interface.Conclusion: The custom-designed molecular imaging device, in combination with ICG, readily allowed differentiation between normal and malignant tissue in a murine model of intrahepatic colorectal metastasis. (C) RSNA, 2014