Generation of iPSC lines from archived non-cryoprotected biobanked dura mater
Generation of iPSC lines from archived non-cryoprotected biobanked dura mater
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DOI：
10.1186/2051-5960-2-4
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发表时间：
2014-01-01
影响因子：
7.1
通讯作者：
Noggle, Scott A.
中科院分区：
文献类型：
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作者：
Sproul, Andrew A.;Vensand, Lauren B.;Noggle, Scott A.
背景资料：来自神经退行性疾病患者的诱导多能干细胞（iPSC）通常缺乏神经病理学证实，这是疾病分类和严重程度分级的金标准。使用具有明确神经病理学诊断的组织将是iPSC的理想来源。对这种方法的挑战是，大多数生物库的脑组织并不意味着生长活细胞，因此没有冷冻在冷冻保护剂如DMS 0.Results的存在下，我们报告了从冷冻的非冷冻保护的硬脑膜组织在-80（圈）C下储存长达11年的iPSC的产生。这个尸检队列包括阿尔茨海默病和其他四种神经退行性疾病的受试者。结论：疾病特异性iPSCs可以从现成的档案生物库组织中产生。这允许快速扩增产生具有确认的病理学的iPSC，以及允许获得已储存的罕见患者变体。
Background: Induced pluripotent stem cells (iPSCs) derived from patients with neurodegenerative disease generally lack neuropathological confirmation, the gold standard for disease classification and grading of severity. The use of tissue with a definitive neuropathological diagnosis would be an ideal source for iPSCs. The challenge to this approach is that the majority of biobanked brain tissue was not meant for growing live cells, and thus was not frozen in the presence of cryoprotectants such as DMSO.Results: We report the generation of iPSCs from frozen non-cryoprotected dural tissue stored at -80(circle)C for up to 11 years. This autopsy cohort included subjects with Alzheimer's disease and four other neurodegenerative diseases.Conclusions: Disease-specific iPSCs can be generated from readily available, archival biobanked tissue. This allows for rapid expansion of generating iPSCs with confirmed pathology as well as allowing access to rare patient variants that have been banked.