Equipment-free and visual detection of multiple biomarkers via an aggregation induced emission luminogen-based paper biosensor
Equipment-free and visual detection of multiple biomarkers via an aggregation induced emission luminogen-based paper biosensor
复制标题
通过基于聚集诱导发射发光剂的纸质生物传感器对多种生物标记物进行无设备视觉检测
DOI：
10.1016/j.bios.2020.112336
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发表时间：
2020-10-01
影响因子：
12.6
通讯作者：
Li, Feng
中科院分区：
文献类型：
--
作者：
Li, Haiyin;Lin, Haiyang;Li, Feng
早期准确的疾病诊断对于挽救患者的生命具有巨大的吸引力，但需要简单、方便、低成本的灵敏检测生物标志物。探索开发用于生物标志物固体、无设备和视觉生物传感的高性能荧光生物传感器迫在眉睫，但面临着巨大的挑战。在此，我们提出了一种全新的荧光系统，将典型的聚集诱导发射染料（TPE-BTD）与多巴胺集成，用于基于靶标诱导催化氧化的多种生物标志物灵敏检测。由TPE-BTD和多巴胺组成的体系发出强荧光；添加辣根过氧化物酶（HRP）或模拟HRP的DNAzyme和H2O2，多巴胺发生显着氧化，生成多巴色素，由于其吸收曲线与TPE-BTD的发射光谱重叠而激发内滤效应（IFE），随后减少荧光发射，并对H2O2和G-四链体DNA表现出快速灵敏的响应。我们进一步将TPE-BTD/多巴胺系统应用于基于靶标启动信号转导的葡萄糖和DNA腺嘌呤甲基化甲基转移酶（Dam MTase）的分析。最后，采用TPE-BTD作为发射体制作纸质生物传感器，基于TPE-BTD的高发射性能，实现了多种生物标志物的固态、免设备、可视化检测，为生物传感器的实际应用开发开辟了新途径。我们期望这种传感概念将有助于开发实用的生物传感器，并且该传感器将在疾病诊断中得到更多应用。
Early and accurate disease diagnosis is of great appeal for saving patients' life, but requires biomarkers to be sensitively detected with simplicity, convenience, and low cost. Exploring the development of a high-performance fluorescence biosensor for biomarkers solid, equipment-free and visual biosensing is highly urgent but faces enormous challenges. Herein, we proposed a brand-new fluorescence system by integrating a typical aggregation induced emission dye (TPE-BTD) with dopamine for multiple biomarkers sensitive detection based on target-induced catalyzing oxidation. The system comprising TPE-BTD and dopamine emits strong fluorescence; with horseradish peroxidase (HRP) or HRP-mimicking DNAzyme and H2O2 being added, significant oxidation on dopamine occurs to generate dopachrome, which actuated the inner filter effect (IFE) due to the overlap of its absorbtion curve and emission spectrum of TPE-BTD, subsequently decreasing fluorescence emission and displaying a rapid and sensitive response to H2O2 and G-quadruplex DNA. We further apply TPE-BTD/dopamine system in analysis of glucose and DNA adenine methylation methyltransferase (Dam MTase) based on target-initiated signal transduction. Finally, TPE-BTD was employed as emitters in fabrication of paper biosensors, which can achieve solid, equipment-free and visual detection of multiple biomarkers based on the high emission performance of TPE-BTD, opening up a new pathway to development of biosensors for practical application. We expect this sensing conception will be helpful in development of practical biosensors, and this sensor will find more applications in disease diagnosis.