Imperfection works: Survival, transmission and persistence in the system of Heliothis virescens ascovirus 3h (HvAV-3h), Microplitis similis and Spodoptera exigua.
Imperfection works: Survival, transmission and persistence in the system of Heliothis virescens ascovirus 3h (HvAV-3h), Microplitis similis and Spodoptera exigua.
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不完美作品：烟夜蛾子囊病毒 3h (HvAV-3h)、类似小蝇和甜菜夜蛾系统中的存活、传播和持久性
DOI：
10.1038/srep21296
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发表时间：
2016-02-16
影响因子：
4.6
通讯作者：
Huang GH
中科院分区：
文献类型：
--
作者：
Li SJ;Hopkins RJ;Zhao YP;Zhang YX;Hu J;Chen XY;Xu Z;Huang GH
囊病毒是一种昆虫特异性的大分子DNA病毒，主要感染粘虫幼虫，田间主要由寄生蜂传播。烟芽夜蛾（Heliothis virescens）3 h型囊状病毒（HvAV-3 h）是近年来从甜菜夜蛾（Spodoptera exigua）上分离到的一种新型病毒。在此，我们报道了一种孤立的内寄生蜂，Microplitis similis，可以在S.实验室里的甜菜幼虫当雌蜂获得病毒并作为媒介时，病毒粒子在产卵器上的存活期为4.1 ± 1.4天。寄生蜂在感染寄主幼虫后仍能产卵，并将病毒传播给健康寄主。感染后3至9天，仅从供体宿主获得病毒。M.蜜蜂在7天前接种病毒的幼虫中产卵。当病毒感染的主机发生在生活史的寄生蜂，它导致1至4日龄的未成熟的寄生蜂死亡的主机，而一小部分的5至6日龄和大多数7日龄的寄生蜂幼虫从病毒感染的主机存活。病毒污染并不降低雌蚊的寿命和繁殖力。相似的
Ascoviruses are insect-specific large DNA viruses that mainly infect noctuid larvae, and are transmitted by parasitoids in the fields. Heliothis virescens ascovirus 3h (HvAV-3h) has been recently isolated from Spodoptera exigua, without parasitoid vector identified previously. Here we report that Microplitis similis, a solitary endoparasitoid wasp, could transmit HvAV-3h between S. exigua larvae in the laboratory. When the female parasitoid wasp acquired the virus and served as a vector, the period of virion viability on the ovipositor was 4.1 ± 1.4 days. Infected host larvae were still acceptable for egg laying by parasitoids, and the parasitoids thereafter transmitted virus to healthy hosts. Virus acquisition occurred only from donor hosts between 3 and 9 days post infection. The peak of virus acquisition (80.9 ± 6.3%) was found when M. similis wasps oviposited in larvae that had been inoculated with the virus 7 days previously. When virus infection of the host took place during the life cycle of the parasitoid wasp, it caused 1- to 4-day-old immature parasitoids death in the host, whilst a small proportion of 5- to 6-day-old and the majority of 7-day-old parasitoids larvae survived from the virus-infected hosts. Viral contamination did not reduce the life span or fecundity of female M. similis.