Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells
Emamectin benzoate induces ROS-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cells
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DOI：
10.1016/j.cbi.2017.06.004
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发表时间：
2017-08-01
影响因子：
5.1
通讯作者：
Huang, Qingchun
中科院分区：
文献类型：
--
作者：
Luan, Shaorong;Yun, Xinming;Huang, Qingchun
甲氨基阿维菌素苯甲酸盐（EMB）是一种新型的大环内酯类杀虫剂，具有高效、选择性的杀虫活性，但其对节肢动物的毒性作用尚不清楚。本研究以粉纹夜蛾Tn 5 B1 -4细胞为模型，研究了EMB诱导活性氧介导的DNA损伤和凋亡的特性。2.5、5、10和15 μ M的EMB作用后，细胞变圆、悬浮、聚集，细胞增殖能力和细胞活力的下降与作用时间呈正相关。EMB对细胞活力的中位抑制浓度（IC 50）为3.72 mM，在72小时的曝光。EMB在15 mM时分别诱导29.8%（24 h）和39.5%（48 h）的细胞凋亡，显示细胞核中染色质凝聚。随着EMB浓度的增加，细胞内ROS含量迅速增加，维生素E预孵育可显著降低细胞内ROS的积累。15 mM EMB处理后，迁移的细胞核出现泪滴状、梨形或大扇形尾，63.1%的γ H2 AX阳性细胞含有4个以上的病灶，并伴有caspase-3的高表达，呈时间依赖性，导致细胞凋亡。这些研究结果有助于从细胞模型上深入理解EMB的细胞毒作用。(C)2017爱思唯尔B. V.保留所有权利。
Emamectin benzoate (EMB), a novel macrocyclic lactone insecticide, possesses high efficacy and beneficial selective toxicity in agriculture, but so far the EMB-induced cytotoxic action in arthropod insect remains unclear. The present studies were carried out to characterize the property of EMB on the induction of reactive oxygen species (ROS)-mediated DNA damage and apoptosis in Trichoplusia Tn5B1-4 cell model. Following the exposure to EMB at 2.5, 5, 10 or 15 mu M, the cells changed to be round, suspended and aggregated, and the decline of cell proliferating ability and cell viability was positively related with the exposure time. Median inhibitory concentration ( IC50) of EMB on cell viability was 3.72 mM during 72 h exposure. Apoptosis was induced in 29.8% ( 24 h) and 39.5% ( 48 h) of the cells by EMB at 15 mM, showing chromatin condensation in nuclei. The content of ROS in the cells increased rapidly as the concentration of EMB increased, and the pre-incubation of the cells with vitamin E significantly reduced the ROS accumulation. In the treatment of 15 mM EMB, the migrated cell nucleus with DNA strand breaks appeared a teardrop, pear-shaped, or large fan-like tail, and 63.1% of gamma H2AX-positive cells contained more than four foci, accompanying with high expression level of caspase-3 in time-dependent manner, which consequently led to cell apoptotic death. These evidences in ROS-mediated DNA damage and cell apoptosis induced by EMB may be helpful for deep understanding the cytotoxic action of EMB based on cell model. (C) 2017 Elsevier B.V. All rights reserved.