Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters
Simultaneous Concentration of Bovine Viruses and Agricultural Zoonotic Bacteria from Water Using Sodocalcic Glass Wool Filters
复制标题
DOI：
10.1007/s12560-014-9159-z
复制
发表时间：
2014-12-01
影响因子：
3.4
通讯作者：
Borchardt, Mark A.
中科院分区：
文献类型：
--
作者：
Abd-Elmaksoud, Sherif;Spencer, Susan K.;Borchardt, Mark A.
人工农田的渗透和径流可导致牲畜病原体进入地下水和地表水。在这里，我们测量了玻璃棉过滤器同时浓缩来自水中的肠道病毒和牛来源细菌的有效性。测定了牛病毒性腹泻病毒1型和2型、牛轮状病毒A组、牛冠状病毒、脊髓灰质炎病毒III、产毒大肠杆菌和空肠弯曲菌三种不同浊度(0.5、215和447NTU)的水中的回收率。将20升脱氯自来水(PH 7)接种试验微生物，然后使用蠕动泵(流量=1升/分钟)通过玻璃棉过滤器。通过牛肉浸膏-甘氨酸缓冲液(pH 9.5)以与样品流动相反的方向从过滤器中洗脱残留的微生物。回收的微生物用定量聚合酶链式反应进行计数，空肠弯曲菌除外，用培养法定量。细菌的平均回收率为55%至33%，病毒的平均回收率为58%至16%。使用自举技术和方差分析相结合的方法，发现在两个最低浑浊度水平下测试的病原菌类型之间的回收效率不同；然而，对于给定的病原菌类型，除空肠弯曲菌外，浊度不影响回收率。玻璃棉过滤是一种同时浓缩几种来自牛的水媒病原体的经济有效的方法，尽管对某些特定的分类群，如牛病毒性腹泻病毒1，回收率可能较低。
Infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. Here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. The recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group A, bovine coronavirus, poliovirus Sabin III, toxigenic Escherichia coli ,and Campylobacter jejuni seeded into water with three different turbidity levels (0.5, 215, and 447 NTU). Twenty liters of dechlorinated tap water (pH 7) were seeded with the test organisms, and then passed through a glass wool filter using a peristaltic pump (flow rate = 1 liter min(-1)). Retained organisms were eluted from the filters by passing beef extract-glycine buffer (pH 9.5) in the direction opposite of sample flow. Recovered organisms were enumerated by qPCR except for C. jejuni, which was quantified by culture. Mean recovery efficiencies ranged from 55 to 33 % for the bacteria and 58 to 16 % for the viruses. Using bootstrapping techniques combined with Analysis of Variance, recovery efficiencies were found to differ among the pathogen types tested at the two lowest turbidity levels; however, for a given pathogen type turbidity did not affect recovery except for C. jejuni. Glass wool filtration is a cost-effective method for concentrating several waterborne pathogens of bovine origin simultaneously, although recovery may be low for some specific taxa such as bovine viral diarrhea virus 1.