PLASTID DYNAMICS DURING SURVIVAL OF DINOPHYSIS CAUDATA WITHOUT ITS CILIATE PREY 1
PLASTID DYNAMICS DURING SURVIVAL OF DINOPHYSIS CAUDATA WITHOUT ITS CILIATE PREY 1
复制标题
没有纤毛虫猎物的甲虫有尾部存活期间的质体动力学 1
作者：
M. Park;Jong Soo Park;Miran Kim;W. Yih
为了生存，海洋鞭毛藻Dinophysis caudata Saville‐Kent必须以可塑纤毛虫Myrionecta rubra （=Mesodinium rubrum）为食，而后者本身就是隐生植物的消费者。然而，尾虫是否有自己的永久叶绿体或保留其纤毛虫猎物的质体仍未解决。此外，D.尾状体（或盗贼体）在没有猎物的情况下持续多长时间并保持光合活性仍然未知。我们在这里解决了这些问题，使用第一个建立的D. caudata培养。对直接来自3种生物（D. caudata， M. rubra, and a cryptophyte）的质体16S rRNA和psbA基因序列的系统发育分析表明，3种生物（D. caudata， M. rubra, and a cryptophyte）的两个基因序列几乎相同，支持D. caudata的质体是kleptoplastids。一项为期3个月的饥饿实验表明，尾尾蟾在没有猎物的情况下可以保持2个月的光合活性。饥饿2个月以上的尾尾藻细胞仍保留了质体16S rRNA基因，但失去了与光合作用相关的基因（即psaA和psbA基因）。然而，当猎物再次出现时，饥饿超过2个月的尾尾尾蟾细胞能够重新获得质体，并慢慢恢复光合作用活动。综上所述，这些结果表明，尾尾草及其质体之间的关系的本质不是永久的细胞获取。D. caudata是一种有趣的原生生物，它代表了一种有趣的光合作用进化适应，也有助于我们更好地理解真核生物的质体进化。
To survive, the marine dinoflagellate Dinophysis caudata Saville‐Kent must feed on the plastidic ciliate Myrionecta rubra (=Mesodinium rubrum), itself a consumer of cryptophytes. Whether D. caudata has its own permanent chloroplasts or retains plastids from its ciliate prey, however, remains unresolved. Further, how long D. caudata plastids (or kleptoplastids) persist and remain photosynthetically active in the absence of prey remains unknown. We addressed those issues here, using the first established culture of D. caudata. Phylogenetic analyses of the plastid 16S rRNA and psbA gene sequences directly from the three organisms (D. caudata, M. rubra, and a cryptophyte) revealed that the sequences of both genes from the three organisms are almost identical to each other, supporting that the plastids of D. caudata are kleptoplastids. A 3‐month starvation experiment revealed that D. caudata can remain photosynthetically active for ∼2 months when not supplied with prey. D. caudata cells starved for more than 2 months continued to keep the plastid 16S rRNA gene but lost the photosynthesis‐related genes (i.e., psaA and psbA genes). When the prey was available again, however, D. caudata cells starved for more than 2 months were able to reacquire plastids and slowly resumed photosynthetic activity. Taken all together, the results indicate that the nature of the relationship between D. caudata and its plastids is not that of permanent cellular acquisitions. D. caudata is an intriguing protist that would represent an interesting evolutionary adaptation with regard to photosynthesis as well as help us to better understand plastid evolution in eukaryotes.
影响因子：
64.8
作者：
Douglas, S;Zauner, S;Maier, UG
通讯作者：
Maier, UG