Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes
Chinese Herbal Formula, Huayu Tongbi Fang, Attenuates Inflammatory Proliferation of Rat Synoviocytes Induced by IL-1β by Regulating Proliferation and Differentiation of T Lymphocytes
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DOI：
10.1155/2020/1706837
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发表时间：
2020-05-20
影响因子：
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通讯作者：
Chai, Lenin
中科院分区：
文献类型：
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作者：
Chen, Hening;Wang, Changzhi;Chai, Lenin
成纤维样滑膜细胞 (FLS) 的炎症增殖和 T 淋巴细胞的功能失衡在类风湿性关节炎 (RA) 的发病机制中发挥着关键作用。化瘀通痹方（HYTB，一种传统草药配方）治疗 RA 的临床疗效已得到验证。本研究旨在探讨HYTB对T淋巴细胞增殖和分化的调节机制，以及HYTB对FLS炎症增殖的抑制作用。 RCS-364（大鼠 FLS）细胞与由 IL-1 β 在 Transwell 室中诱导的大鼠脾淋巴细胞共培养。冻干 HYTB 粉末处理后，使用流式细胞术测量 T 细胞亚群的百分比和 FLS 的凋亡率。此外，使用蛋白质印迹对 NF-κ B 和 JAK/STAT 信号通路关键分子的蛋白表达进行定量。使用酶联免疫吸附测定法测量粒细胞-巨噬细胞集落刺激因子（GM-CSF）。结果表明，HYTB可以通过诱导细胞凋亡来抑制FLSs的炎症增殖。此外，HYTB治疗可以干预T淋巴细胞的增殖和分化，并调节NF-κB和JAK/STAT细胞信号通路中关键分子的蛋白表达。此外，它还可以通过抑制 T 细胞和 FLS 产生 GM-CSF 来抑制 FLS 激活。因此，HYTB方剂应作为现代补充和替代疗法中对抗RA的传统药物。
The inflammatory proliferation of fibroblast-like synoviocytes (FLSs) and functional imbalances in T lymphocytes play critical roles in the pathogenesis of rheumatoid arthritis (RA). The clinical efficacy of Huayu Tongbi Fang (HYTB, a traditional herbal formula) in RA treatment has been validated. In this study, we aimed to explore the regulatory mechanisms of HYTB on the proliferation and differentiation of T lymphocytes, and the inhibitory effect of HYTB on inflammatory proliferation of FLSs. The RCS-364 (Rat FLSs) cells were cocultured with rat splenic lymphocytes that were induced by interleukin-1 beta in Transwell chambers. After freeze-dried HYTB powder treatment, the percentage of T-cell subset and apoptosis rates of FLSs were measured using flow cytometry. Furthermore, protein expression of key molecules of NF-kappa B and JAK/STAT signaling pathways was quantified using Western blot. The granulocyte-macrophage colony-stimulating factor (GM-CSF) was measured using enzyme-linked immunosorbent assay. The results showed that HYTB could inhibit the inflammatory proliferation of FLSs through inducing cell apoptosis. Additionally, HYTB treatment could intervene in the proliferation and differentiation of T lymphocytes and regulate protein expression of key molecules in NF-kappa B and JAK/STAT cell signaling pathways. Moreover, it could inhibit FLS activation by suppressing GM-CSF production by T cells and FLSs. Therefore, the HYTB formula should be used as a traditional medicine against RA in modern complementary and alternative therapies.