Identification and profiling of Bactrocera dorsalis microRNAs and their potential roles in regulating the developmental transitions of egg hatching, molting, pupation and adult eclosion.
Identification and profiling of Bactrocera dorsalis microRNAs and their potential roles in regulating the developmental transitions of egg hatching, molting, pupation and adult eclosion.
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DOI：
10.1016/j.ibmb.2020.103475
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发表时间：
2020-10
影响因子：
3.8
通讯作者：
Qiang Zhang;W. Dou;Zhong-Hao Song;T. Jin;Guorui Yuan;K. De Schutter;G. Smagghe;Jinjun Wang
中科院分区：
文献类型：
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作者：
Qiang Zhang;W. Dou;Zhong-Hao Song;T. Jin;Guorui Yuan;K. De Schutter;G. Smagghe;Jinjun Wang
microRNA（miRNAs）是一类内源性小分子非编码RNA（18-25 nt），通过转录后调节基因表达，参与发育、肿瘤、免疫、凋亡和宿主-微生物相互作用等多种生理过程。在这项研究中，我们测量了小RNA的轮廓的发育过渡的东方果蝇桔小实蝇从卵孵化，蜕皮，化蛹，成虫羽化。我们鉴定了250种miRNAs，包括83种已知的miRNAs和167种新的miRNAs，以及47种isomiRNAs。此外，我们还鉴定了在发育转变过程中差异表达的miRNAs。有趣的是，miR-309簇，miR-2簇/家族和let-7簇在这些差异表达的miRNA中，分别表明在卵孵化，蜕皮和化蛹/成虫羽化的调节中的作用。此外，对蛹期14种高表达miRNA的时间表达模式的详细分析揭示了三种类型的表达谱。此外，在3龄幼虫中注射miR-100模拟物导致化蛹和成虫羽化率显著降低，而注射miR-317模拟物导致化蛹率显著降低和化蛹时间缩短，表明miR-100和miR-317参与化蛹过程。最后，在蛹中注射miR-100/miR-285模拟物或Eschomir导致羽化率显著降低和部分羽化表型的患病率显著增加，这意味着miR-100和miR-285参与成虫羽化过程。这项研究确定了参与这种重要的全代谢模型和害虫insectB的转变的关键miRNAs。dorsalis从卵孵化到成虫羽化，为探索miRNAs在昆虫胚后发育中的调控作用提供了有用的资源。
MicroRNAs (miRNAs) are endogenous small noncoding RNAs (18–25 nt) that are involved in many physiological processes including development, cancer, immunity, apoptosis and host-microbe interactions through post-transcriptional regulation of gene expression. In this study, we measured the profile of small RNAs over the developmental transitions of the oriental fruit fly Bactrocera dorsalis from egg hatching, molting, and pupation to adult eclosion. We identified 250 miRNAs, including 83 known and 167 novel miRNAs, and 47 isomiRNAs. In addition, we identified the miRNAs differentially expressed over the developmental transitions. Interestingly, the miR-309 cluster, the miR-2 cluster/family and the let-7 cluster were among these differentially expressed miRNAs, suggesting a role in the regulation of egg hatching, molting and pupation/adult eclosion, respectively. Moreover, a detailed analysis of the temporal expression patterns of 14 highly expressed miRNAs in the pupal stage revealed three types of expression profiles. Furthermore, injection of a miR-100 mimic in the 3rd instar larvae resulted in a significant decrease in pupation and adult eclosion rates, whereas injection of a miR-317 antagomir resulted in a significant decrease in the pupation rate and a decrease in the pupation time, indicating that miR-100 and miR-317 are involved in the process of pupation. Finally, injection of a miR-100/miR-285 mimic or antagomir in pupae resulted in a significant decrease in the eclosion rate and a significant increase in the prevalence of a partial eclosion phenotype, implying the involvement of miR-100 and miR-285 in the process of adult eclosion. This study identified critical miRNAs involved in the transitions of this important holometabolic model and pest insectB. dorsalisfrom egg hatching to adult eclosion, thus providing a useful resource for exploring the regulatory role of miRNAs during insect post-embryonic development.