DETERMINATION OF REDUCED SCATTERING AND ABSORPTION-COEFFICIENTS BY A SINGLE CHARGE-COUPLED-DEVICE ARRAY MEASUREMENT .1. COMPARISON BETWEEN EXPERIMENTS AND SIMULATIONS

DETERMINATION OF REDUCED SCATTERING AND ABSORPTION-COEFFICIENTS BY A SINGLE CHARGE-COUPLED-DEVICE ARRAY MEASUREMENT .1. COMPARISON BETWEEN EXPERIMENTS AND SIMULATIONS
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DOI:
10.1117/12.204798
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发表时间:
1995-07-01
影响因子:
1.3
通讯作者:
DEHALLER, EB
DEHALLER, EB
中科院分区:
工程技术4区
文献类型:
--
作者:
MARQUET, P;BEVILACQUA, F;DEHALLER, EB

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发展了一种由光的空间分布精确确定混浊介质的吸收系数和折合散射系数的方法。它允许在体外对样品进行局部测量,并且只需要由线阵CCD探测器进行一次测量。散射光的强度分布以强度的最大值和半峰值的全宽度为特征。这些参数在理论上与吸收系数和折合散射系数有关。理论方法基于蒙特卡罗模拟,用来预测输出表面的光强分布。考虑了边界反射以及源和探测器的特性。对于小于6个输运平均自由程的厚度,根据使用的是Mie相函数还是Henyey-Greenstein相函数(具有相同的各向异性因子),发现了显著的差异。这有助于确定相似关系的有效范围。在微球悬浮液上进行的模拟和实验之间发现了非常好的一致性(误差通常小于5%,最大为15%)。
A precise method to determine the absorption and reduced scattering coefficients of turbid media from the spatial distribution of light has been developed. it allows in vitro local measurements on samples, and needs only one measurement performed by a linear CCD detector. The intensity profile of the scattered light is characterized by the maximum of the intensity and the full width at half maximum. These parameters have been related theoretically to the absorption and reduced scattering coefficients. The theoretical approach is based on Monte Carlo simulations, which are used to predict the intensity profile at the output surface. The boundary reflections and the source and detector characteristics have been taken into account. For a thickness lower than 6 transport mean free paths, significant differences have been found depending on whether a Mie or a Henyey-Greenstein phase function (with the same anisotropic factor) is used. This is of help in the determination of the validity range of the similarity relations. Very good agreement (error typically less than 5%, maximal 15%) has been found between simulations and experiments performed on microsphere suspensions.