γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology
γ-H2AX as a biomarker for DNA double-strand breaks in ecotoxicology
复制标题
DOI：
10.1016/j.ecoenv.2014.03.035
复制
发表时间：
2014-07-01
影响因子：
6.8
通讯作者：
Garaj-Vrhovac, Vera
中科院分区：
文献类型：
--
作者：
Geric, Marko;Gajski, Goran;Garaj-Vrhovac, Vera
DNA损伤反应蛋白的可视化使得能够间接测量DNA损伤。在DNA双链断裂（DSB）发生后不久，预计会形成γ-H2 AX组蛋白变体。本文综述了γ-H2 AX焦点试验在评估环境污染物（包括细胞抑制药物）的遗传毒性方面的潜在用途，因为标准方法可能不够灵敏，无法检测低环境浓度此类药物的破坏作用。这些化合物不断释放到环境中，可能对水质、水生生物以及最终对人类健康构成威胁。我们对文献的回顾表明，这种方法可以用于生物监测和风险评估的水生系统受废水的生产，使用和处置的细胞抑制药物。(C)2014爱思唯尔公司All rights reserved.
The visualisation of DNA damage response proteins enables the indirect measurement of DNA damage. Soon after the occurrence of a DNA double-strand break (DSB), the formation of gamma-H2AX histone variants is to be expected. This review is focused on the potential use of the gamma-H2AX foci assay in assessing the genotoxicity of environmental contaminants including cytostatic pharmaceuticals, since standard methods may not be sensitive enough to detect the damaging effect of low environmental concentrations of such drugs. These compounds are constantly released into the environment, potentially representing a threat to water quality, aquatic organisms, and, ultimately, human health. Our review of the literature revealed that this method could be used in the biomonitoring and risk assessment of aquatic systems affected by wastewater from the production, usage, and disposal of cytostatic pharmaceuticals. (C) 2014 Elsevier Inc. All rights reserved.