EPILEPTIFORM ACTIVITY IN MICROCULTURES CONTAINING SMALL NUMBERS OF HIPPOCAMPAL-NEURONS
EPILEPTIFORM ACTIVITY IN MICROCULTURES CONTAINING SMALL NUMBERS OF HIPPOCAMPAL-NEURONS
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DOI：
10.1152/jn.1990.64.5.1390
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发表时间：
1990-11-01
影响因子：
2.5
通讯作者：
FURSHPAN, EJ
中科院分区：
文献类型：
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作者：
SEGAL, MM;FURSHPAN, EJ
1.微培养物中含有少量海马神经元。神经元生长在胶质细胞上，胶质细胞附着在胶原蛋白或钯片上。微培养物下面的琼脂糖层阻止了附近微培养物之间形成连接。2.神经元在每个微培养物内形成强的化学突触连接，具有单突触的快速兴奋性、快速抑制性和缓慢抑制性突触作用。3.只有两个神经元的小网络产生的癫痫样活动与含有数千个神经元的大规模培养物中观察到的癫痫样活动非常相似。当在突触活性阻断剂（犬尿氨酸和升高的Mg 2+）中生长数周的微培养物被冲洗掉这些阻断剂时，观察到癫痫样活动。4.这种微培养技术允许在一个简化的系统中研究癫痫样活动，并有助于分析癫痫样活动背后的突触活动。
1. Microcultures were grown containing small numbers of hippocampal neurons. The neurons grew on glial cells attached to patches of either collagen or palladium. A layer of agarose underlying the microcultures prevented connections from forming between nearby microcultures. 2. Neurons formed strong chemical synaptic connections within each microculture, with monosynaptic fast-excitatory, fast-inhibitory, and slow-inhibitory synaptic actions. 3. Small networks with as few as two neurons generated epileptiform activity that closely resembled the epileptiform activity seen in mass cultures containing thousands of neurons. The epileptiform activity was observed when microcultures that were grown for weeks in blockers of synaptic activity (kynurenate and elevated Mg2+) were washed free of these blockers. 4. Such a microculture technique allows study of epileptiform activity in a simplified system and facilitates analysis of the synaptic actions underlying the epileptiform activity.