Identification of oocyte progenitor cells in the zebrafish ovary.
Identification of oocyte progenitor cells in the zebrafish ovary.
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DOI：
10.1007/978-1-61779-980-8_12
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发表时间：
2012-01-01
期刊：
影响因子：
--
通讯作者：
Draper, Bruce W
中科院分区：
文献类型：
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作者：
Draper, Bruce W
斑马鱼全年繁殖，雌性斑马鱼一生中能产下数千个卵。这种惊人的生育能力是由于成年卵巢含有早衰性卵母细胞祖细胞，称为卵原细胞，在成年生活中不断产生新的卵母细胞。卵母细胞祖细胞可以根据其表达Vasa和核形态特征来识别。因此，斑马鱼卵巢为研究脊椎动物卵母细胞产生的遗传调控提供了一个独特而强大的系统。本文提出了一种简便、准确的全载共聚焦免疫荧光法鉴定斑马鱼卵巢卵母细胞祖细胞的方法。
Zebrafish breed year round and females are capable of producing thousands of eggs during their lifetime. This amazing fecundity is due to the fact that the adult ovary, contains premeiotic oocyte progenitor cells, called oogonia, which produce a continuous supply of new oocytes throughout adult life. Oocyte progenitor cells can be easily identified based on their expression of Vasa, and their characteristic nuclear morphology. Thus, the zebrafish ovary provides a unique and powerful system to study the genetic regulation of oocyte production in a vertebrate animal. A method is presented here for identifying oocyte progenitor cells in the zebrafish ovary using whole-mount confocal immunofluorescence that is simple and accurate.