Applications of Yeast Surface Display for Protein Engineering.
Applications of Yeast Surface Display for Protein Engineering.
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DOI：
10.1007/978-1-4939-2748-7_8
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Cochran JR
中科院分区：
文献类型：
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作者：
Cherf GM;Cochran JR
通过与丰富的细胞壁蛋白的基因融合在酿酒酵母表面上展示重组蛋白的方法，称为酵母表面展示或简单地称为酵母展示的技术，已经成为用于广谱生物技术和生物医学应用的有价值的蛋白质工程工具。本文综述了利用酵母展示技术研究蛋白质的亲和力、稳定性和酶活性。每个蛋白质工程目标的策略和例子进行了讨论。此外，还简要介绍了酵母展示技术的其他应用，包括蛋白质表位作图、蛋白质相互作用的鉴定以及展示蛋白在工业和医学中的应用。
The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.