Erosion from Staphylococcus aureus biofilms grown under physiologically relevant fluid shear forces yields bacterial cells with reduced avidity to collagen.
Erosion from Staphylococcus aureus biofilms grown under physiologically relevant fluid shear forces yields bacterial cells with reduced avidity to collagen.
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在生理相关的流体剪切力下生长的金黄色葡萄球菌生物膜的侵蚀产生对胶原蛋白的亲和力降低的细菌细胞。
DOI：
10.1128/aem.01319-06
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发表时间：
2007
影响因子：
4.4
通讯作者：
Ross,JuliaM
中科院分区：
文献类型：
--
作者：
Ymele-Leki,Patrick;Ross,JuliaM
据估计，65%的传染病与细菌生物膜的存在有关。生物膜生成的浮游细胞通过血管内空间的细菌接种感染部位，促进血液传播的继发感染部位。为了研究早期脱离事件在引发继发性感染中的潜在作用，我们研究了金黄色葡萄球菌浮游细胞从生物膜上侵蚀的表型属性，以及胶原粘附素（CNA）的表达。s。金黄色葡萄球菌与骨髓炎和脓毒性关节炎的发生有关。在本研究中，我们关注的是CNA表达对onS的影响。在生理相关剪切力作用下，金黄色葡萄球菌与固定胶原的体外粘附。不同于s。在悬浮液中生长的金黄色细胞，受侵蚀的浮游细胞表现出不变的和较低的有效粘附率，而不管它们起源于的生物膜的年龄。这些结果与CNA的表面表达水平直接相关。然而，随后的分析显示，悬浮细胞引发的生物膜和生物脱落浮游细胞引发的次生生物膜之间没有质的差异。综上所述，我们的研究结果表明，尽管它们的CNA表达水平较低，但S。从生物膜脱落的金黄色浮游细胞保留转移性扩散和继发感染的能力。这些发现表明，需要更好地了解侵蚀浮游细胞的表型特性，这可能导致针对继发性感染的新治疗策略。
An estimated 65% of infective diseases are associated with the presence of bacterial biofilms. Biofilm-issued planktonic cells promote blood-borne, secondary sites of infection by the inoculation of the infected sites with bacteria from the intravascular space. To investigate the potential role of early detachment events in initiating secondary infections, we studied the phenotypic attributes ofStaphylococcus aureusplanktonic cells eroding from biofilms with respect to expression of the collagen adhesin, CNA. The collagen-binding abilities ofS. aureushave been correlated to the development of osteomyelitis and septic arthritis. In this study, we focused on the impact of CNA expression onS. aureusadhesion to immobilized collagen in vitro under physiologically relevant shear forces. In contrast to the growth phase-dependent adhesion properties characteristic ofS. aureuscells grown in suspension, eroding planktonic cells expressed invariant and lower effective adhesion rates regardless of the age of the biofilm from which they originated. These results correlated directly with the surface expression level of CNA. However, subsequent analysis revealed no qualitative differences between biofilms initiated with suspension cells and secondary biofilms initiated with biofilm-shed planktonic cells. Taken together, our findings suggest that, despite their low levels of CNA expression,S. aureusplanktonic cells shed from biofilms retain the capacity for metastatic spread and the initiation of secondary infection. These findings demonstrate the need for a better understanding of the phenotypic properties of eroding planktonic cells, which could lead to new therapeutic strategies to target secondary infections.