Comparison of the Degree of Autophagy in Neonatal Rat Cardiomyocytes and H9c2 Cells Exposed to Hypoxia/Reoxygenation
Comparison of the Degree of Autophagy in Neonatal Rat Cardiomyocytes and H9c2 Cells Exposed to Hypoxia/Reoxygenation
复制标题
缺氧/复氧条件下新生大鼠心肌细胞与H9c2细胞自噬程度的比较
DOI：
10.7754/clin.lab.2013.130521
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发表时间：
2014-01-01
影响因子：
0.7
通讯作者：
Chen, Aihua
中科院分区：
文献类型：
--
作者：
Cao, Xueming;Wang, Xianbao;Chen, Aihua
Background: Hypoxia/reoxygenation (H/R) is an important in vitro model for exploring the molecular mechanisms and functions of autophagy during myocardial ischemia/reperfusion (I/R). Neonatal rat cardiomyocytes (NRCM) and H9c2 cells are widely used to study H/R.Methods: The degree of autophagy in NRCM and H9c2 cells exposed to H/R was assessed by detecting the markers of autophagy, Beclin-1 and LC3II. Autophagosomes were confirmed in both NRCM and H9c2 cells exposed to H/R using MDC staining and TEM.Results: The expression levels of Beclin-1 and LC3II were significantly increased in NRCM under H/R conditions (p < 0.05 vs. control). In contrast, the expression levels of Beclin-1 and LC3II were significantly reduced in H9c2 cells exposed to H/R (p < 0.05 vs. control). The fluorescence intensity and the number of MDC-labeled particles were greater in NRCM exposed to H/R, compared to H9c2 cells (p
