Activation-induced expression of human programmed Death-1 gene in T-Lymphocytes
Activation-induced expression of human programmed Death-1 gene in T-Lymphocytes
复制标题
DOI：
10.1006/excr.1997.3493
复制
发表时间：
1997-04-10
影响因子：
3.7
通讯作者：
Finger, LR
中科院分区：
文献类型：
--
作者：
Vibhakar, R;Juan, G;Finger, LR
程序性死亡-1 （PD-1）基因是免疫球蛋白超家族的一员。在小鼠t细胞杂交瘤细胞系和小鼠胸腺细胞中，PD-1 mRNA的表达已被证明与激活诱导的凋亡相关。在这里，我们报告了人类同源物hPD-1的表达似乎与T淋巴细胞的激活而不是凋亡相关。我们观察到在phorbol酯（12-O-tetradecanoylphorbol 13-acetate， TPA）诱导的Jurkat细胞分化过程中，hPD-1 mRNA和蛋白水平呈时间依赖性上调。在凝集素刺激的人外周血单核细胞活化过程中也可诱导人PD-1蛋白。此外，TPA刺激Jurkat细胞诱导hPD-1的酪氨酸磷酸化，推测是在其细胞质尾部信号转导基序上，这些数据表明hPD-1在t淋巴细胞的激活和分化过程中起作用。(C) 1997学术出版社。
The Programmed Death-1 (PD-1) gene is a member of the immunoglobulin superfamily of genes. Murine PD-1 mRNA expression has been shown to correlate with activation-induced apoptosis in a mouse T-cell hybridoma cell line and in murine thymocytes. Here we report that expression of the human homolog, hPD-1, seems to correlate with activation of T lymphocytes rather than apoptosis. We observed a time-dependent upregulation of hPD-1 mRNA and protein levels in Jurkat cells during phorbol ester (12-O-tetradecanoylphorbol 13-acetate, TPA)-induced differentiation. Human PD-1 protein was also induced during lectin-stimulated activation of human peripheral blood mononuclear cells. Additionally, TPA stimulation of Jurkat cells induces tyrosine phosphorylation of hPD-1, putatively on its cytoplasmic tail signal transduction motif, These data suggest a role for hPD-1 during activation and differentiation of T-lymphocytes. (C) 1997 Academic Press.