Choline acetyltransferase: purification and immunohistochemical localization.
Choline acetyltransferase: purification and immunohistochemical localization.
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胆碱乙酰转移酶：纯化和免疫组织化学定位。
DOI：
10.1016/0024-3205(84)90419-3
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发表时间：
1984
期刊：
影响因子：
6.1
通讯作者：
J. H. Peng
中科院分区：
文献类型：
--
作者：
P. Mcgeer;E. Mcgeer;J. H. Peng
胆碱的米氏常数估计值为0.4-1 mM，乙酰辅酶A的米氏常数估计值为10-20 uM。在胆碱能神经元中，胆碱通过高亲和力摄取系统提供，而乙酰辅酶A通过涉及克雷布斯循环中间体的一系列复杂反应在细胞内产生。体内通常存在过量的酶，并且随着胆碱能神经元a~ iv~（I）的增加，合成量增加。体内的速率控制机制仍然不清楚。
Estimated Michaelis constants are of the order of 0.4-1 mM for choline and 10-20 uM for acetyl CoA. In cholinergic neurons, choline is supplied via a high affinity uptake system, while acetylcoenzyme A is generated within the cell via a complex series of reactions involving Krebs cycle intermediates. Excess enzyme is normally present in vivo and the amount of synthesis is increased with cholinergic neuronal a~ iv~(I). The rate controlling mechanisms in vivo are still not understood.