Relationship between Thy‐1 expression and cell‐cycle distribution in human bone marrow hematopoietic progenitors
Relationship between Thy‐1 expression and cell‐cycle distribution in human bone marrow hematopoietic progenitors
复制标题
DOI：
10.1002/ajh.20362
复制
发表时间：
2005-07
影响因子：
12.8
通讯作者：
H. Takeda;Masuji Yamamoto;N. Morita;T. Tanizawa
中科院分区：
文献类型：
--
作者：
H. Takeda;Masuji Yamamoto;N. Morita;T. Tanizawa
对造血干细胞（HSC）的Thy-1表达与细胞周期分布之间关系的分析表明，新鲜分离的CD 34 highCD 38 −flt-3−Lin−群体的Thy-1+和Thy-1−亚群主要处于G 0/G1期，基本上保持静止，而在细胞因子刺激6天后，Thy-1+亚群进入循环状态，而Thy-1-亚群再次保持静止。Thy-1抗原的表达导致CD 34 highCD 38-flt-3-Lin-Thy-1+-以及CD 34 highCD 38-flt-3-Lin-Thy-1--细胞启动培养物中循环细胞百分比急剧增加。CD 34 highCD 38 −flt-3−Lin−群体的Thy-1+亚群存在于新鲜分离的CD 34 highCD 38 −flt-3−Lin− Thy-1+组分中，在6天内失去Thy-1表达，并再表达Thy-1 2天。细胞周期分析表明，这个独特的亚群含有丰富的S/G2 M细胞。因此，Thy-1表达似乎是靶向HSC的细胞周期阶段的指标，这可能在最适合基因转移的细胞亚群中起作用。Am. J. Hematol. 79：187-193，2005.© 2005 Wiley利斯公司
Analysis of the relationship between Thy‐1 expression and cell‐cycle distribution of hematopoietic stem cells (HSCs) showed that freshly isolated Thy‐1+ and Thy‐1− subsets of the CD34highCD38−flt‐3−Lin− population were predominantly in G0/G1 phase and remained essentially quiescent, whereas after 6 days of cytokine stimulation, the Thy‐1+ subset of the population entered the cycling state while the Thy‐1− subset again remained quiescent. Expression of Thy‐1 antigen resulted in a drastic increase in the percentage of cycling cells in CD34highCD38‐flt‐3‐Lin‐Thy‐1+‐ as well as CD34highCD38−flt‐3−Lin− Thy‐1−‐cell‐initiated cultures. The Thy‐1+ subset of the CD34highCD38−flt‐3−Lin− population exists in the freshly isolated CD34highCD38−flt‐3−Lin− Thy‐1+ fraction, loses Thy‐1 expression during 6 days, and re‐expresses Thy‐1 for an additional 2 days. Cell‐cycle analysis demonstrated that this unique subset contains abundant S/G2M cells. Thus, Thy‐1 expression appears to be an indicator of cell‐cycle phase in targeting HSC, which might serve in the cell subset best suited for gene transfer. Am. J. Hematol. 79:187–193, 2005. © 2005 Wiley‐Liss, Inc.