Influence of intravenously administered leptin on nitric oxide production, renal hemodynamics and renal function in the rat
Influence of intravenously administered leptin on nitric oxide production, renal hemodynamics and renal function in the rat
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DOI：
10.1016/j.regpep.2004.02.012
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发表时间：
2004-08-15
影响因子：
--
通讯作者：
Zwirska-Korczala, K
中科院分区：
文献类型：
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作者：
Beltowski, J;Jochem, J;Zwirska-Korczala, K
我们研究了瘦素对大鼠全身一氧化氮（NO）产生、动脉压、肾血流动力学和肾排泄功能的影响。静脉注射瘦素(1mg/kg)，注射后2-10分钟测量平均动脉压(MAP)、心率(HR)、肾血流量(RBF)和肾皮质血流量(RCBF)。连续七个30分钟收集尿液，并在瘦素施用后15、45、75、105、135、165和195分钟抽取血样。瘦素对 MAP、HR、RBF、RCBF 和肌酐清除率没有影响，但尿量增加 37.8%（0-30 分钟）、32.4%（31-60 分钟）和 27.0%（61-90 分钟），尿钠排泄增加 175.8%（0-30 分钟）、136.4%（31-60 分钟）和124.2%（61-90 分钟）。相反，瘦素对钾和磷酸盐的排泄没有影响。注射瘦素后，NO 代谢物、亚硝酸盐 + 硝酸盐 (NO.) 的血浆浓度在 15、45、75 和 105 分钟分别增加了 27.7%、178.1%、156.4% 和 58.7%。瘦素使尿NO排泄量增加241.6%（0-30分钟）、552.6%（31-60分钟）、430.7%（61-90分钟）和88.9%（91-120分钟）。这伴随着血浆和尿循环 GMP 的增加。这些数据表明，瘦素刺激全身NO产生，但对动脉压和肾血流动力学没有影响。 (C) 2004 Elsevier B.V. 保留所有权利。
We investigated the effect of leptin on systemic nitric oxide (NO) production, arterial pressure, renal hemodynamics and renal excretory function in the rat. Leptin (I mg/kg) was injected intravenously and mean arterial pressure (MAP), heart rate (HR), renal blood flow (RBF) and renal cortical blood flow (RCBF), were measured for 2 10 min after injection. Urine was collected for seven consecutive 30-min periods and blood samples were withdrawn at 15, 45, 75, 105, 135, 165 and 195 min after leptin administration. Leptin had no effect on MAP, HR, RBF, RCBF and creatinine clearance, but increased urine output by 37.8% (0-30 min), 32.4% (31-60 min) and 27.0% (61-90 min), as well as urinary sodium excretion by 175.8% (0-30 min), 136.4% (31-60 min) and 124.2% (61-90 min). In contrast, leptin had no effect on potassium and phosphate excretion. Plasma concentration of NO metabolites, nitrites + nitrates (NO.), increased following leptin injection at 15, 45, 75 and 105 min by 27.7%, 178.1%, 156.4% and 58.7%, respectively. Leptin increased urinary NO, excretion by 241.6% (0-30 min), 552.6% (31-60 min), 430.7% (61-90 min) and 88.9% (91 - 120 min). This was accompanied by increase in plasma and urinary cyclic GMP. These data indicate that leptin stimulates systemic NO production but has no effect on arterial pressure and renal hemodynamics. (C) 2004 Elsevier B.V. All rights reserved.