Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA
Rapid identification and phylogenetic classification of diverse bacterial pathogens in a multiplexed hybridization assay targeting ribosomal RNA
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DOI：
10.1038/s41598-019-40792-3
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发表时间：
2019-03-14
影响因子：
4.6
通讯作者：
Hung, Deborah T.
中科院分区：
文献类型：
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作者：
Bhattacharyya, Roby P.;Walker, Mark;Hung, Deborah T.
快速细菌鉴定仍然是传染病诊断中的一个关键挑战。我们开发了一种新颖的分子方法，通过单一、简单的测定来检测和鉴定多种细菌病原体，在不需要扩增或酶学的快速荧光杂交测定中利用核糖体 RNA 的保守性、丰度和丰富的系统发育内容。在来自 4 个门 64 个物种的 117 个分离株中，该检测在物种水平上以 > 89% 的准确度识别细菌，在科水平上以 100% 的准确度识别细菌，从而实现所有关键的临床区别。在对主要临床样本（包括痰液、血培养物和脓液）的初步研究中，鉴定出了来自 5 个不同门的细菌。
Rapid bacterial identification remains a critical challenge in infectious disease diagnostics. We developed a novel molecular approach to detect and identify a wide diversity of bacterial pathogens in a single, simple assay, exploiting the conservation, abundance, and rich phylogenetic content of ribosomal RNA in a rapid fluorescent hybridization assay that requires no amplification or enzymology. Of 117 isolates from 64 species across 4 phyla, this assay identified bacteria with >89% accuracy at the species level and 100% accuracy at the family level, enabling all critical clinical distinctions. In pilot studies on primary clinical specimens, including sputum, blood cultures, and pus, bacteria from 5 different phyla were identified.