Epidemiology of multiple Plasmodium falciparum infections -: 6.: Multiple Plasmodium falciparum infections in Tanzanian infants
Epidemiology of multiple Plasmodium falciparum infections -: 6.: Multiple Plasmodium falciparum infections in Tanzanian infants
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DOI：
10.1016/s0035-9203(99)90324-3
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发表时间：
1999-02-01
影响因子：
2.2
通讯作者：
Beck, HP
中科院分区：
文献类型：
--
作者：
Felger, I;Smith, T;Beck, HP
对来自99名坦桑尼亚婴儿的配对血液样本进行了分析，以检查生命第一年期间恶性疟原虫的感染动态。感染的寄生虫基因分型的裂殖子表面蛋白2的多态性基因的聚合酶链反应扩增和随后的分析，根据所得的限制性片段长度多态性模式。在一项平行病例对照研究中，同样的样本作为对照，在发烧发作期间从每个孩子身上采集额外的血液样本。分析了并发感染人数（多重性）与年龄和发病率的关系，并将结果与同一时间在同一村庄对2至7岁年龄较大的儿童进行的类似研究的结果进行了比较。平均2感染基因型每阳性血液样本从社区调查相比，在年龄较大的儿童，并没有显着的年龄依赖性的多重性在第一年的生活。发热病例的多重感染也与年龄无关。在婴儿中，多重性与寄生虫密度和临床疟疾风险呈正相关，与年龄较大的儿童（>2岁）的情况相反。这些发现有助于了解疟疾的感染动力学、预防和半免疫力的发展。
Paired blood samples from 99 Tanzanian infants were analysed to examine the infection dynamics of Plasmodium falciparum during the first year of life. Infecting parasites were genotyped by polymerase chain reaction amplification of the polymorphic gene for the merozoite surface protein 2 and subsequent analysis according to the resulting restriction fragment length polymorphism pattern. The same samples served as controls in a parallel case-control study for which an additional blood sample was taken from each child during a fever episode. The relationship of the number of concurrent infections (multiplicity) with age and morbidity was analysed and results were compared to those of a similar study on older children between 2 and 7 years of age, carried out in the same village at the same time. The mean of 2 infecting genotypes per positive blood sample from community surveys was low compared to that in older children, and there was no significant age-dependency of multiplicity within the first year of life. Multiplicity of infection in fever cases was also independent of age. In infants, multiplicity was positively associated with parasite density and risk of clinical malaria, in contrast to the situation in older children (>2 years). The findings help in the understanding of infection dynamics, premunition, and development of semi-immunity in malaria.