A spectrin membrane skeleton of the Golgi complex
A spectrin membrane skeleton of the Golgi complex
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DOI：
10.1016/s0167-4889(98)00054-8
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发表时间：
1998-08-14
影响因子：
5.1
通讯作者：
Nelson, WJ
中科院分区：
文献类型：
--
作者：
Beck, KA;Nelson, WJ
高尔基体定位的膜细胞骨架的存在已被发现的两个主要组成部分的血影蛋白膜骨架，血影蛋白和锚蛋白，与高尔基体复合体的鉴定。高尔基血影蛋白用血影蛋白的红系同种型的P-亚基特异性抗体（β 1 Sigma 1）鉴定。该抗体识别220 kDa多肽，该多肽定位于高尔基复合体的离散区域，并以布雷菲德菌素A敏感的方式与高尔基膜结合。已经鉴定了高尔基体锚蛋白的两种同种型：119 kDa形式（Ank（G119）），其代表最近克隆的神经系统新锚蛋白Ank（G）的截短的、选择性剪接的同种型，以及更大的195 kDa锚蛋白（Ank（195）），其与红细胞锚蛋白的抗体交叉反应。由这些独特的膜骨架异构体组成的高尔基体局部膜骨架可以发挥多种重要功能，包括维持高尔基体结构组织和在高尔基体隔室内形成离散的膜结构域。(C)1998 Elsevier Science B. V.保留所有权利。
The existence of a Golgi-localized membrane cytoskeleton has been revealed by the identification of two major components of the spectrin membrane skeleton, spectrin and ankyrin, that associate with the Golgi complex. Golgi spectrin was identified with an antibody specific for the P-subunit of the erythroid isoform of spectrin (beta 1 Sigma 1). This antibody recognizes a 220 kDa polypeptide that localizes to discrete regions of the Golgi complex and associates with Golgi membranes in a Brefeldin A sensitive manner. Two isoforms of Golgi ankyrin have been identified: a 119 kDa form (Ank(G119)) which represents a truncated, alternatively spliced isoform of a recently cloned novel ankyrin of the nervous system Ank(G), and a larger 195 kDa ankyrin (Ank(195)) that cross-reacts with antibodies to erythrocyte ankyrin. A Golgi localized membrane skeleton composed of these unique membrane skeleton isoforms could serve a variety of important functions, including the maintenance of Golgi structural organization and the formation of discrete membrane domains within Golgi compartments. (C) 1998 Elsevier Science B.V. All rights reserved.