Gold Nanoring Arrays for Near Infrared Plasmonic Biosensing
Gold Nanoring Arrays for Near Infrared Plasmonic Biosensing
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DOI：
10.1007/s11468-013-9657-0
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发表时间：
2014-08-01
期刊：
影响因子：
3
通讯作者：
Corn, Robert M.
中科院分区：
文献类型：
--
作者：
Toma, Mana;Cho, Kyunghee;Corn, Robert M.
金纳米环阵列表面，表现出强烈的局部表面等离子体共振（LSPR）在近红外（NIR）波长从1.1到1.6 μ m的高度敏感的实时折射率生物传感器。通过将金纳米环纳米级电沉积到大面积（平方厘米）上的光刻图案化纳米孔阵列导电表面上来产生具有可调直径、宽度和间距的金纳米环阵列。通过FT-NIR透射光谱测量监测LSPR峰的位移，确定金纳米环阵列的体折射率灵敏度高达3，780 cm（-1）/折射率单位。作为第一个应用，用实时LSPR峰位移测量监测多巴胺从水溶液在纳米环传感器表面上形成聚多巴胺薄膜的表面聚合反应。为了证明金纳米环阵列用于LSPR生物传感的实用性，监测DNA官能化的金纳米颗粒在互补的DNA官能化的金纳米环阵列上的杂交吸附。还检测到DNA修饰的金纳米颗粒吸附到用仅含有0.5%互补DNA的混合DNA单层修饰的纳米环阵列上;该相对表面覆盖度对应于通过从50 pM溶液的杂交吸附检测DNA。
Gold nanoring array surfaces that exhibit strong localized surface plasmon resonances (LSPR) at near infrared (NIR) wavelengths from 1.1 to 1.6 mu m were used as highly sensitive real-time refractive index biosensors. Arrays of gold nanorings with tunable diameter, width, and spacing were created by the nanoscale electrodeposition of gold nanorings onto lithographically patterned nanohole array conductive surfaces over large areas (square centimeters). The bulk refractive index sensitivity of the gold nanoring arrays was determined to be up to 3,780 cm(-1)/refractive index unit by monitoring shifts in the LSPR peak by FT-NIR transmittance spectroscopy measurements. As a first application, the surface polymerization reaction of dopamine to form polydopamine thin films on the nanoring sensor surface from aqueous solution was monitored with the real-time LSPR peak shift measurements. To demonstrate the utility of the gold nanoring arrays for LSPR biosensing, the hybridization adsorption of DNA-functionalized gold nanoparticles onto complementary DNA-functionalized gold nanoring arrays was monitored. The adsorption of DNA-modified gold nanoparticles onto nanoring arrays modified with mixed DNA monolayers that contained only 0.5 % complementary DNA was also detected; this relative surface coverage corresponds to the detection of DNA by hybridization adsorption from a 50 pM solution.