Complete, Annotated Sequence of the Pseudorabies Virus Genome
Complete, Annotated Sequence of the Pseudorabies Virus Genome
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DOI：
10.1128/jvi.78.4.2166.2004
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发表时间：
2004-02
影响因子：
5.4
通讯作者：
B. Klupp;C. Hengartner;T. Mettenleiter;L. Enquist
中科院分区：
文献类型：
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作者：
B. Klupp;C. Hengartner;T. Mettenleiter;L. Enquist
我们已经获得了伪狂犬病病毒(PRV)的完整DNA序列，这是一种甲型疱疹病毒，也被称为Aujeszky病病毒或猪疱疹病毒1，使用来自六个不同毒株(Kaplan、Becker、Rice、Indiana-Funkhauser、NIA-3和TNL)的序列片段。组装的PRV基因组序列由143,461个核苷酸组成。正如预期的那样，它与预测的基因排列、基因组大小和限制性内切酶消化模式相匹配。有70多个开放阅读框与相关甲型疱疹病毒的同源物被鉴定，没有一个是PRV独有的。用计算机预测程序鉴定了PRV基因组中的RNA聚合酶II转录调控元件，包括核心启动子、剪接位点和多聚腺苷化位点。预测的转录起始和终止位点与实验确定的转录起始和终止位点之间的相关性很好。转录控制结构有三个关键特征：核心转录元件在基因之间共享，产生不同的转录本和大量的共末端转录本；双功能转录元件，产生从头到尾的转录本；以及短重复序列，可以作为绝缘体来对抗错误终止的转录本。这些特征中的许多在甲型疱疹病毒亚家族中是保守的，并对基因阵列分析具有重要意义。
We have obtained the complete DNA sequence of pseudorabies virus (PRV), an alphaherpesvirus also known as Aujeszky's disease virus or suid herpesvirus 1, using sequence fragments derived from six different strains (Kaplan, Becker, Rice, Indiana-Funkhauser, NIA-3, and TNL). The assembled PRV genome sequence comprises 143,461 nucleotides. As expected, it matches the predicted gene arrangement, genome size, and restriction enzyme digest patterns. More than 70 open reading frames were identified with homologs in related alphaherpesviruses; none were unique to PRV. RNA polymerase II transcriptional control elements in the PRV genome, including core promoters, splice sites, and polyadenylation sites, were identified with computer prediction programs. The correlation between predicted and experimentally determined transcription start and stop sites was excellent. The transcriptional control architecture is characterized by three key features: core transcription elements shared between genes, yielding divergent transcripts and a large number of coterminal transcripts; bifunctional transcriptional elements, yielding head-to-tail transcripts; and short repetitive sequences that could function as insulators against improperly terminated transcripts. Many of these features are conserved in the alphaherpesvirus subfamily and have important implications for gene array analyses.