Self-assembled monolayers of thiol-end-labeled DNA at mercury electrodes
Self-assembled monolayers of thiol-end-labeled DNA at mercury electrodes
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DOI：
10.1021/la061424v
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发表时间：
2006-07-18
期刊：
影响因子：
3.9
通讯作者：
Palecek, Emil
中科院分区：
文献类型：
--
作者：
Ostatna, Veronika;Palecek, Emil
我们首次发现硫醇末端标记的寡核苷酸在汞电极上形成自组装单分子膜(SAM)。胞嘧啶和汞-S键还原引起的伏安信号变化表明，随着HS-(TTC)(7)浓度的增加，HS-(TTC)(7)分子在电极上的位置发生了变化，形成了直立的分子。这种SAM起到了绝缘体的作用，不允许电子在[Ru(NH3)(6)](3+)和电极之间转移。观察到硫醇DNA和无硫醇DNA的不同吸附模式。
We show for the first time that thiol-end-labeled oligodeoxynucleotides form self-assembled monolayer (SAM) at Hg electrodes. Changes in voltammetric signals due to cytosine and Hg-S bond reduction indicated changes in positioning of the HS-(TTC)(7) molecules at the electrode with increasing HS-(TTC)(7) concentration from lying flat with respect to SAMs, forming upright-standing molecules. This SAM behaved as an insulator not allowing electron transfer between [Ru(NH3)(6)](3+) and the electrode. Different adsorption modes of thiolated and thiol-free DNAs were observed.