Egg fucose sulfate polymer, sialoglycan, and speract all trigger the sea urchin sperm acrosome reaction.
Egg fucose sulfate polymer, sialoglycan, and speract all trigger the sea urchin sperm acrosome reaction.
复制标题
鸡蛋岩藻糖硫酸聚合物、唾液酸聚糖和speract都会引发海胆精子顶体反应。
DOI：
10.1016/s0006-291x(02)00941-5
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发表时间：
2002
影响因子：
3.1
通讯作者：
Vacquier,VictorD
中科院分区：
文献类型：
--
作者：
Hirohashi,Noritaka;Vacquier,VictorD
卵子周围的大分子物质可诱导精子的顶体反应。在海胆中，三种卵冻（EJ）分子：岩藻糖硫酸酯聚合物（FSP），唾液酸聚糖（SG）和精子介导触发AR的离子通量。SG和speract在没有FSP的情况下是无诱导的。Speract在AR诱导中的作用是有争议的。在这里，我们表明，speract增强FSP诱导的AR在pH 7.0，0.1 pH单位低于天然海水。在pH 7.0时，FSP、SG和speract的混合物产生最大AR诱导所必需的细胞内pH升高。每种EJ组分可以介导不同的细胞内pH控制机制，并且所有三种可以协同作用以增加允许AR诱导的细胞内pH。精子肽是一个古老的家族。虽然在今天的海水pH值为1.8的激活环核苷酸介导的途径很重要，speract可能是更重要的AR诱导在pH1.7的古海洋。
Macromolecules surrounding eggs induce the acrosome reaction (AR) of spermatozoa. In sea urchins, three egg jelly (EJ) molecules: a fucose sulfate polymer (FSP), a sialoglycan (SG), and speract mediate ionic fluxes triggering the AR. SG and speract are noninductive without FSP. Speract's role in AR induction is controversial. Here we show that speract potentiates the FSP-induced AR at pH 7.0, ∼1 pH unit lower than natural seawater. At pH 7.0, a mixture of FSP, SG, and speract produces the intracellular pH increase necessary for maximum AR induction. Each EJ component may mediate a distinct intracellular pH control mechanism, and all three may function synergistically to increase the intracellular pH permitting AR induction. Speract peptides are an ancient family. Although important for activating cyclic nucleotide-mediated pathways in today's seawater of pH ∼8, speract may have been more important in AR induction in the paleo-ocean of pH ∼7.