Topology control of human fibroblast cells monolayer by liquid crystal elastomer
Topology control of human fibroblast cells monolayer by liquid crystal elastomer
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DOI：
10.1126/sciadv.aaz6485
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发表时间：
2020-05-01
期刊：
影响因子：
13.6
通讯作者：
Lavrentovich, Oleg D.
中科院分区：
文献类型：
--
作者：
Turiv, Taras;Krieger, Jess;Lavrentovich, Oleg D.
活组织中的真核细胞形成具有空间上变化的取向顺序的动态模式，其影响重要的生理过程，如凋亡和细胞迁移。挑战在于如何将预先设计的定向顺序映射到生长的组织上。在这里，我们展示了一种方法来生产细胞单层的人皮肤成纤维细胞与预先设计的取向模式和拓扑缺陷，使用光取向的液晶弹性体（LCE），在水性介质中各向异性膨胀。铭刻到LCE的图案被组织单层复制，并导致细胞表型、表面密度和数量密度波动的强烈空间变化。由各向异性表面锚定允许估计的组织的弹性特性的缺陷对固有的活性物质的解约束动力学被抑制。所展示的模式化LCE方法具有控制活组织中细胞的集体行为、细胞分化和组织形态发生的潜力。
Eukaryotic cells in living tissues form dynamic patterns with spatially varying orientational order that affects important physiological processes such as apoptosis and cell migration. The challenge is how to impart a predesigned map of orientational order onto a growing tissue. Here, we demonstrate an approach to produce cell monolayers of human dermal fibroblasts with predesigned orientational patterns and topological defects using a photoaligned liquid crystal elastomer (LCE) that swells anisotropically in an aqueous medium. The patterns inscribed into the LCE are replicated by the tissue monolayer and cause a strong spatial variation of cells phenotype, their surface density, and number density fluctuations. Unbinding dynamics of defect pairs intrinsic to active matter is suppressed by anisotropic surface anchoring allowing the estimation of the elastic characteristics of the tissues. The demonstrated patterned LCE approach has potential to control the collective behavior of cells in living tissues, cell differentiation, and tissue morphogenesis.