EXPRESSION OF NIDOGEN AND LAMININ IN BASEMENT-MEMBRANES DURING MOUSE EMBRYOGENESIS AND IN TERATOCARCINOMA CELLS
EXPRESSION OF NIDOGEN AND LAMININ IN BASEMENT-MEMBRANES DURING MOUSE EMBRYOGENESIS AND IN TERATOCARCINOMA CELLS
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DOI：
10.1016/0012-1606(85)90491-9
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发表时间：
1985-01-01
影响因子：
2.7
通讯作者：
TIMPL, R
中科院分区：
文献类型：
--
作者：
DZIADEK, M;TIMPL, R
巢蛋白和层粘连蛋白定位于小鼠发育的着床前阶段。层粘连蛋白已经存在于细胞表面的2细胞阶段，而巢蛋白是第一次检测到紧凑的8至16细胞阶段桑椹胚。巢蛋白和层粘连蛋白共定位于囊胚期和着床后基底膜。组织提取物和细胞培养基的免疫印迹分析表明，150 kDa形式的巢蛋白作为最大的和主要的形式在所有组织检查。放射性标记的巢蛋白和层粘连蛋白合成的Reichert的膜共沉淀的抗体对每个抗原，表明复合物形成原位。通过放射免疫测定法在组织的6M盐酸胍提取物中测定等摩尔量的层粘连蛋白和巢蛋白，进一步表明化学计量复合物。然而，在组织和细胞培养基中发现巢蛋白水平低于层粘连蛋白。当用维甲酸和二丁酰cAMP刺激F9细胞分化时，发现巢蛋白增加不到2倍，而层粘连蛋白分泌增加30倍。
Nidogen and laminin were localized at preimplantation stages of mouse development by immunofluorescence. Laminin was already present on the cell surface at the 2-cell stage, while nidogen was first detectable on compacted 8- to 16-cell stage morulae. Nidogen and laminin colocalized at the blastocyst stage and in postimplantation basement membranes. Immunoblot analyses of tissue extracts and cell culture media indicated the 150-kDa form of nidogen as the largest and predominant form in all tissues examined. Radiolabeled nidogen and laminin synthesized by Reichert''s membrane were coprecipitated by antibodies against each antigen, indicating complex formation in situ. Equimolar amounts of laminin and nidogen were determined in 6 M guanidine.cntdot.HCl extracts of tissues by radioimmunoassays, further indicating stoichiometric complexes. However, lower levels of nidogen than laminin were found in tissue and cell culture media. A less than 2-fold increase in nidogen was found when F9 cells were stimulated to differentiate with retionic acid and dibutyryl cAMP, compared to a 30-fold increase in laminin secretion.