Growth factor-stimulated generation of new cortical tissue and functional recovery after stroke damage to the motor cortex of rats
Growth factor-stimulated generation of new cortical tissue and functional recovery after stroke damage to the motor cortex of rats
复制标题
DOI：
10.1038/sj.jcbfm.9600402
复制
发表时间：
2007-05-01
影响因子：
6.3
通讯作者：
Weiss, Samuel
中科院分区：
文献类型：
--
作者：
Kolb, Bryan;Morshead, Cindi;Weiss, Samuel
最近的研究表明，增殖在成人前脑室下区增加，以响应前脑中风和脑室内输注的生长因子增强这种反应。生长因子输注再生受损的运动皮层和促进中风后运动功能恢复的潜力尚未得到研究。在这里，我们报告说，脑室内输注表皮生长因子和促红细胞生成素一起，但不是单独，促进实质性的再生受损的大脑皮层和逆转损伤的自发和熟练的运动任务，在大鼠模型中风。即使在卒中诱导的损伤后生长因子给药延迟7天，皮质再生和功能恢复也会发生。细胞追踪表明，起源于前脑室管膜下区的神经前体对再生皮层的贡献。引人注目的是，去除再生的皮质组织逆转了生长因子诱导的功能恢复。这些发现表明，生长因子的特定组合可以动员内源性成体神经干细胞，以促进脑卒中后皮质组织的再生长和功能恢复。
Recent studies suggest that proliferation in the adult forebrain subventricular zone increases in response to a forebrain stroke and intraventricular infusions of growth factors enhance this response. The potential for growth factor infusions to regenerate the damaged motor cortex and promote recovery of motor function after stroke has not been examined. Here, we report that intraventricular infusions of epidermal growth factor and erythropoietin together, but not individually, promote substantial regeneration of the damaged cerebral cortex and reverse impairments in spontaneous and skilled motor tasks, in a rat model of stroke. Cortical regeneration and functional recovery occurred even when growth factor administration was delayed for up to 7 days after the stroke-induced lesion. Cell tracking demonstrated the contribution of neural precursors originating in the forebrain subventricular zone to the regenerated cortex. Strikingly, removal of the regenerated cortical tissue reversed the growth factor induced functional recovery. These findings reveal that specific combinations of growth factors can mobilize endogenous adult neural stem cells to promote cortical tissue re-growth and functional recovery after stroke.