Magnetically-activated, nanostructured cellulose for efficient capture of circulating tumor cells from the blood sample of head and neck cancer patients
Magnetically-activated, nanostructured cellulose for efficient capture of circulating tumor cells from the blood sample of head and neck cancer patients
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DOI：
10.1016/j.carbpol.2023.121418
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发表时间：
2023-09-28
影响因子：
11.2
通讯作者：
Quadir，Mohiuddin
中科院分区：
文献类型：
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作者：
Hazra，Raj Shankar;Kale，Narendra;Quadir，Mohiuddin
在这份报告中，评估了纤维素纳米晶体（CNC）和纳米纤维（CNF）从头颈癌（HNC）患者的血液样本中捕获循环肿瘤细胞（CTC）的相对效率。CTC的检测和计数对于监测癌症进展至关重要。这两种类型的纳米结构纤维素与上皮细胞粘附分子（EpCAM）抗体和氧化铁纳米颗粒进行化学修饰。EpCAM抗体促进CTC的接合，促进纤维素笼结构内的包埋。另一方面，氧化铁纳米颗粒使得笼可通过使用磁体活化以捕获和分离截留的CTC。在头颈癌（HNC）患者的血液样品中显示了网络结构的效率。观察到，位于具有抗EpCAM的CNC或CNF内的羟基的化学官能化程度决定了系统与CTC相互作用的效率。此外，我们的研究结果表明，非柔性支架的纳米晶体更有效地与CTC的纤维CNF支架的相互作用。来自CNC的网络结构证明了与商业标准OncoDiscover®相当的CTC捕获效率。这项工作的成果将提供纤维素材料的化学设计原理，用于构建“真实的时间”监测癌症进展的负担得起的平台。
In this report, the relative efficiency of cellulose nanocrystals (CNCs) and nanofibers (CNFs) to capture circulating tumor cells (CTCs) from the blood sample of head and neck cancer (HNC) patients was evaluated. Detection and enumeration of CTCs are critical for monitoring cancer progression. Both types of nanostructured cellulose were chemically modified with Epithelial Cell Adhesion Molecule (EpCAM) antibody and iron oxide nanoparticles. The EpCAM antibody facilitated the engagement of CTCs, promoting entrapment within the cellulose cage structure. Iron oxide nanoparticles, on the other hand, rendered the cages activatable via the use of a magnet for the capture and separation of entrapped CTCs. The efficiency of the network structures is shown in head and neck cancer (HNC) patients' blood samples. It was observed that the degree of chemical functionalization of hydroxyl groups located within the CNCs or CNFs with anti-EpCAM determined the efficiency of the system's interaction with CTCs. Further, our result indicated that inflexible scaffolds of nanocrystals interacted more efficiently with CTCs than that of the fibrous CNF scaffolds. Network structures derived from CNCs demonstrated comparable CTC capturing efficiency to commercial standard, OncoDiscover®. The output of the work will provide the chemical design principles of cellulosic materials intended for constructing affordable platforms for monitoring cancer progression in ‘real time’.