Mesenchymal stem cell growth behavior on micro/nano hierarchical surfaces of titanium substrates
Mesenchymal stem cell growth behavior on micro/nano hierarchical surfaces of titanium substrates
复制标题
间充质干细胞在钛基底微纳分级表面上的生长行为
DOI：
10.1016/j.colsurfb.2015.01.048
复制
发表时间：
2015-03-01
影响因子：
5.8
通讯作者：
Cai, Kaiyong
中科院分区：
文献类型：
--
作者：
Shen, Xinkun;Ma, Pingping;Cai, Kaiyong
骨科植入物的表面形貌在调节周围骨组织的骨形成中起着重要作用。为了研究钛（Ti）基底表面形貌对间充质干细胞（MSC）细胞行为的影响，通过溶胶-凝胶法和旋涂技术在微结构钛（Micro-Ti）基底上制备了一系列微/纳米分级结构。采用扫描电子显微镜 (SEM)、表面轮廓仪、X 射线衍射 (XRD)、X 射线光电子能谱 (XPS) 和水接触角测量来证明微结构表面上存在各种纳米尺寸 TiO2 颗粒（分别为 20 nm、40 nm 和 80 nm）的微/纳米分级结构的成功制造。提出了微纳米分级结构的形成机制。此外，在体外细胞和分子水平上评估了这些分层结构对 MSC 生长行为的影响。结果证实，与其他小颗粒（20 nm和40 nm）相比，大颗粒（80 nm）的微/纳米分级结构极大地促进了MSC的增殖和分化。该研究为潜在骨科应用的分层结构钛植入物的制造提供了一种替代方案。 (C) 2015 Elsevier B.V. 保留所有权利。
Surface topography of an orthopedic implant plays an essential role in the regulation of bone formation with surrounding bone tissue. To investigate the effects of surface topography of titanium (Ti) substrates on cellular behavior of mesenchymal stem cells (MSCs), a series of micro/nano hierarchical structures were fabricated onto micro-structured titanium (Micro-Ti) substrates via a sol-gel method with spin-coat technique. Scanning electron microscopy (SEM), surface profiler, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and water contact angle measurement were employed to certify the successful fabrication of micro/nano hierarchical structures with the presence of various nano-sized TiO2 grains (20 nm, 40 nm and 80 nm, respectively) onto micro-structured surfaces. The formation mechanism of the micro/nano hierarchical structures was proposed. Moreover, the effects of those hierarchical structures on the growth behavior of MSCs were evaluated both on cellular and molecular levels in vitro. The results confirmed that micro/nano hierarchical structures with large grains (80 nm) greatly promoted the proliferation and differentiation of MSCs comparing with other small grains (20 nm and 40 nm). The study provides an alternative for the fabrication of hierarchically structured Ti implants for potential orthopedic application. (C) 2015 Elsevier B.V. All rights reserved.