Expression of the RNA recognition motif-containing protein SEB-4 during Xenopus embryonic development
Expression of the RNA recognition motif-containing protein SEB-4 during Xenopus embryonic development
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DOI：
10.1016/s0925-4773(00)00284-7
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发表时间：
2000-06-01
影响因子：
2.6
通讯作者：
Bouwmeester, T
中科院分区：
文献类型：
--
作者：
Fetka, I;Radeghieri, A;Bouwmeester, T
RNA结合蛋白在基因表达的转录后调控中起着关键作用。在这里，我们介绍了XSEB-4的分子克隆和时空表达，XSEB-4编码一个含有单个RNA识别基序(RRM)的假定RNA结合蛋白。XSEB-4与哺乳动物SEB-4蛋白有60%-65%的同源性。XSEB-4在母体中强烈表达。合子转录是在近轴中胚层的原肠胚早期启动的，注定会产生体节。在原肠发育和神经分化过程中，Xseb-4在闪族人的旁轴中胚层中的表达集中在XmyoD表达区域。随着发育的进行，Xseb-4在心脏原基和晶状体囊泡中也开始表达。在心脏中的表达仅限于心肌。因此，含有RRM的推测的RNA结合蛋白XSEB-4在非洲爪哇胚胎发育过程中存在差异表达。(C)2000爱思唯尔爱尔兰科学有限公司。保留所有权利。
RNA binding proteins play key roles in the post-transcriptional regulation of gene expression. Here we present the molecular cloning and spatio-temporal expression of Xseb-4, which codes for a putative RNA binding protein containing a single RNA recognition motif (RRM). XSEB-4 shares 60-65% identity with the mammalian SEB-4 proteins. Xseb-4 is strongly expressed maternally. Zygotic transcription is initiated in the early gastrula embryo in paraxial mesoderm that is fated to give :rise to somites. During the course of gastrulation and neurulation Xseb-4 expression in semitic paraxial mesoderm is centered within the XmyoD expression domain. As development proceeds Xseb-4 expression is in addition initiated in the cardiac primordium and the lens vesicle. In the heart expression is confined to the myocardium. Thus, the RRM-containing putative RNA binding protein XSEB-4 is differentially expressed during embryonic development in Xenopus. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.