CHARACTERIZATION OF SEVERAL CLONAL LINES OF CULTURED LEYDIG TUMOR-CELLS - GONADOTROPIN RECEPTORS AND STEROIDOGENIC RESPONSES
CHARACTERIZATION OF SEVERAL CLONAL LINES OF CULTURED LEYDIG TUMOR-CELLS - GONADOTROPIN RECEPTORS AND STEROIDOGENIC RESPONSES
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DOI：
10.1210/endo-108-1-88
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发表时间：
1981-01-01
期刊：
影响因子：
4.8
通讯作者：
ASCOLI, M
中科院分区：
文献类型：
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作者：
ASCOLI, M
已经建立了几种培养的睾丸间质肿瘤细胞的克隆系，并在促性腺激素受体和类固醇产生方面进行了表征。尽管从M5480 P肿瘤中分离的细胞具有功能性hCG [人绒毛膜促性腺激素]受体，但建立的5个克隆系中只有2个显示出结合显著量的hCG。在这些克隆中，类固醇的产生可以被hCG、霍乱毒素和8-Br-cAMP刺激到相同的程度。其他3个克隆有少量的hCG，对激素的反应是类固醇生成的轻微增加。类固醇的产生，但是，显着刺激霍乱毒素或8-Br-cAMP。由新鲜分离的细胞和2个克隆产生的类固醇的比较揭示了类固醇生成途径的一些变化。最明显的变化是培养的细胞合成20 α的能力增加。二氢孕酮（20 α-羟基孕甾-4-烯-3-酮）。这些克隆系可为研究促性腺激素的作用和调节间质细胞分化功能的表达提供一个合适的模型系统。
Several clonal lines of cultured Leydig tumor cells have been established and characterized in terms of gonadotropin receptors and steroid production. Although freshly isolated cells derived from the M5480P tumor have functional hCG [human chorionic gonadotropin] receptors, only 2 of the 5 clonal lines established were shown to bind significant quantitites of hCG. In these clones, steroid production can be stimulated to the same extent by hCG, cholera toxin and 8-Br-cAMP. The other 3 clones had a small amount of hCG and respond to the hormone with a marginal increase in steroidogenesis. Steroid production, however, is significantly stimulated by cholera toxin or 8-Br-cAMP. A comparison of the steroids produced by freshly isolated cells and 2 of the clones revealed some changes in the steroidogenic pathway. The most obvious change is an increase in the ability of the cultured cells to synthesize 20.alpha.-dihydroprogesterone (20.alpha.-hydroxypregn-4-en-3-one). These clonal lines may provide a suitable model system for the study of gonadotropin actions and regulation of the expression of differentiated functions of Leydig cells.